Literature DB >> 3054485

Repair of double-strand breaks in plasmid DNA in the yeast Saccharomyces cerevisiae.

J R Perera1, A V Glasunov, V M Glaser, A V Boreiko.   

Abstract

We studied the repair of double-strand breaks (DSB) in plasmid DNA introduced into haploid cells of the yeast Saccharomyces cerevisiae. The efficiency of repair was estimated from the frequency of transformation of the cells by an autonomously replicated linearized plasmid. The frequency of "lithium" transformation of Rad+ cells was increased greatly (by 1 order of magnitude and more) compared with that for circular DNA if the plasmid was initially linearized at the XhoI site within the LYS2 gene. This effect is due to recombinational repair of the plasmid DNA. Mutations rad52, rad53, rad54 and rad57 suppress the repair of DSB in plasmid DNA. The kinetics of DSB repair in plasmid DNA are biphasic: the first phase is completed within 1 h and the second within 14-18 h of incubating cells on selective medium.

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Year:  1988        PMID： 3054485     DOI： 10.1007/bf00339611

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  10 in total

1.  Mitotic intragenic recombination in the yeast Saccharomyces: marker-effects on conversion and reciprocity of recombination.

Authors:  Y O Chernoff; O V Kidgotko; O Demberelijn; I L Luchnikova; S P Soldatov; V M Glazer; D A Gordenin
Journal:  Curr Genet       Date:  1984-12       Impact factor: 3.886

2.  A rapid alkaline extraction method for the isolation of plasmid DNA.

Authors:  H C Birnboim
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

3.  Repair of double-strand breaks in a temperature conditional radiation-sensitive mutant of Saccharomyces cerevisiae.

Authors:  M Budd; R K Mortimer
Journal:  Mutat Res       Date:  1982-01       Impact factor: 2.433

4.  The repair of double-strand breaks in the nuclear DNA of Saccharomyces cerevisiae and its genetic control.

Authors:  M A Resnick; P Martin
Journal:  Mol Gen Genet       Date:  1976-01-16

5.  Transformation of yeast with linearized plasmid DNA. Formation of inverted dimers and recombinant plasmid products.

Authors:  S Kunes; D Botstein; M S Fox
Journal:  J Mol Biol       Date:  1985-08-05       Impact factor: 5.469

6.  The use of plasmid DNA to probe DNA repair functions in the yeast Saccharomyces cerevisiae.

Authors:  C I White; S G Sedgwick
Journal:  Mol Gen Genet       Date:  1985

7.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

8.  Yeast transformation: a model system for the study of recombination.

Authors:  T L Orr-Weaver; J W Szostak; R J Rothstein
Journal:  Proc Natl Acad Sci U S A       Date:  1981-10       Impact factor: 11.205

9.  Cloning arg3, the gene for ornithine carbamoyltransferase from Saccharomyces cerevisiae: expression in Escherichia coli requires secondary mutations; production of plasmid beta-lactamase in yeast.

Authors:  M Crabeel; F Messenguy; F Lacroute; N Glansdorff
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

10.  Yeast recombination: the association between double-strand gap repair and crossing-over.

Authors:  T L Orr-Weaver; J W Szostak
Journal:  Proc Natl Acad Sci U S A       Date:  1983-07       Impact factor: 11.205
  10 in total
  11 in total

1.  RAD51 is required for the repair of plasmid double-stranded DNA gaps from either plasmid or chromosomal templates.

Authors:  S Bärtsch; L E Kang; L S Symington
Journal:  Mol Cell Biol       Date:  2000-02       Impact factor: 4.272

Review 2.  The biology of radioresistance: similarities, differences and interactions with drug resistance.

Authors:  S N Powell; E H Abraham
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

3.  Plasmid-mediated induction of recombination in yeast.

Authors:  R Silberman; M Kupiec
Journal:  Genetics       Date:  1994-05       Impact factor: 4.562

4.  Genetic control of plasmid DNA double-strand gap repair in yeast, Saccharomyces cerevisiae.

Authors:  V M Glaser; A V Glasunov; G G Tevzadze; J R Perera; S V Shestakov
Journal:  Curr Genet       Date:  1990-07       Impact factor: 3.886

5.  Disruption of the RAD52 gene alters the spectrum of spontaneous SUP4-o mutations in Saccharomyces cerevisiae.

Authors:  B A Kunz; M G Peters; S E Kohalmi; J D Armstrong; M Glattke; K Badiani
Journal:  Genetics       Date:  1989-07       Impact factor: 4.562

6.  Influence of non-homology between recombining DNA sequences on double-strand break repair in Saccharomyces cerevisiae.

Authors:  A Glasunov; M Frankenberg-Schwager; D Frankenberg
Journal:  Mol Gen Genet       Date:  1995-04-10

7.  Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting.

Authors:  Anamarija Štafa; Marina Miklenić; Bojan Zunar; Berislav Lisnić; Lorraine S Symington; Ivan-Krešimir Svetec
Journal:  DNA Repair (Amst)       Date:  2014-08-02

8.  The use of a double-marker shuttle vector to study DNA double-strand break repair in wild-type and radiation-sensitive mutants of the yeast Saccharomyces cerevisiae.

Authors:  B Jha; F Ahne; F Eckardt-Schupp
Journal:  Curr Genet       Date:  1993 May-Jun       Impact factor: 3.886

9.  Further characterization of the yeast pso4-1 mutant: interaction with rad51 and rad52 mutants after photoinduced psoralen lesions.

Authors:  M A de Morais; E J Vicente; J Brozmanova; A C Schenberg; J A Henriques
Journal:  Curr Genet       Date:  1996-02       Impact factor: 3.886

10.  The DNA damage checkpoint pathways exert multiple controls on the efficiency and outcome of the repair of a double-stranded DNA gap.

Authors:  Edwin Haghnazari; Wolf-Dietrich Heyer
Journal:  Nucleic Acids Res       Date:  2004-08-10       Impact factor: 16.971
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