| Literature DB >> 30542983 |
Anton Baysa1,2, Anton Fedorov3,4, Kirill Kondratov3, Arno Ruusalepp5, Sarkis Minasian3, Michael Galagudza3, Maxim Popov3, Dmitry Kurapeev3, Alexey Yakovlev3, Guro Valen1,2, Anna Kostareva3, Jarle Vaage6, Kåre-Olav Stensløkken7,8.
Abstract
During heart surgery with cardiopulmonary bypass (CPB), the release of mitochondrial (mtDNA) and nuclear DNA (nDNA) and their association to extracellular vesicles were investigated. In patients undergoing elective coronary artery bypass grafting (CABG, n = 12), blood was sampled before, during, and after surgery from peripheral artery, pulmonary artery, and the coronary sinus. Plasma was separated in three fractions: microvesicles, exosomes, and supernatant. mtDNA and nDNA were measured by qPCR. mtDNA and nDNA levels increased after start of surgery, but before CPB, and increased further during CPB. mtDNA copy number was about 1000-fold higher than nDNA. mtDNA was predominantly localized to the vesicular fractions in plasma, whereas nDNA was predominantly in the supernatant. The amount of free mtDNA increased after surgery. There was no net release or disappearance of DNAs across the pulmonary, systemic, or coronary circulation. Extracellular DNAs, in particular mtDNA, may be important contributors to the whole-body inflammation during CPB.Entities:
Keywords: Cardiac surgery; Exosomes; Extracellular DNA; Microvesicles
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Year: 2018 PMID： 30542983 DOI： 10.1007/s12265-018-9848-3
Source DB: PubMed Journal: J Cardiovasc Transl Res ISSN： 1937-5387 Impact factor: 4.132