Literature DB >> 30542657

CRISPR-Induced TMPRSS2-ERG Gene Fusions in Mouse Prostate Organoids.

Else Driehuis1,2, Hans Clevers1,3.   

Abstract

TMPRSS2-ERG fusions are common genetic events in prostate cancer. Until now, this genetic alteration was modelled by ERG overexpression. In this short communication, we report the creation of mouse prostate organoids that have undergone gene fusion through a CRISPR/Cas9-based strategy. The genetic fusion of TMPRSS2 and ERG results in ERG overexpression. This effect is androgen receptor-mediated, as expression of the fusion transcript can be restored to wildtype ERG levels by treatment with the androgen receptor antagonist Nilutamide.

Entities:  

Keywords:  CRISPR/Cas9; Genetic engineering; Organoids; TMPRSS2-ERG

Year:  2017        PMID： 30542657      PMCID： PMC6287496     

Source DB:  PubMed          Journal:  JSM Biotechnol Biomed Eng        ISSN： 2333-7117


  30 in total

1.  Genome engineering using the CRISPR-Cas9 system.

Authors:  F Ann Ran; Patrick D Hsu; Jason Wright; Vineeta Agarwala; David A Scott; Feng Zhang
Journal:  Nat Protoc       Date:  2013-10-24       Impact factor: 13.491

2.  TMPRSS2:ERG fusion by translocation or interstitial deletion is highly relevant in androgen-dependent prostate cancer, but is bypassed in late-stage androgen receptor-negative prostate cancer.

Authors:  Karin G Hermans; Ronald van Marion; Herman van Dekken; Guido Jenster; Wytske M van Weerden; Jan Trapman
Journal:  Cancer Res       Date:  2006-11-15       Impact factor: 12.701

3.  A variant TMPRSS2 isoform and ERG fusion product in prostate cancer with implications for molecular diagnosis.

Authors:  Jacques Lapointe; Young H Kim; Melinda A Miller; Chunde Li; Gulsah Kaygusuz; Matt van de Rijn; David G Huntsman; James D Brooks; Jonathan R Pollack
Journal:  Mod Pathol       Date:  2007-03-02       Impact factor: 7.842

4.  Pleiotropic biological activities of alternatively spliced TMPRSS2/ERG fusion gene transcripts.

Authors:  Jianghua Wang; Yi Cai; Wendong Yu; Chengxi Ren; David M Spencer; Michael Ittmann
Journal:  Cancer Res       Date:  2008-10-15       Impact factor: 12.701

5.  TMPRSS2- driven ERG expression in vivo increases self-renewal and maintains expression in a castration resistant subpopulation.

Authors:  Orla M Casey; Lei Fang; Paul G Hynes; Wassim G Abou-Kheir; Philip L Martin; Heather S Tillman; Gyorgy Petrovics; Hibah O Awwad; Yvona Ward; Ross Lake; Luhua Zhang; Kathleen Kelly
Journal:  PLoS One       Date:  2012-07-30       Impact factor: 3.240

6.  Identification of multipotent luminal progenitor cells in human prostate organoid cultures.

Authors:  Wouter R Karthaus; Phillip J Iaquinta; Jarno Drost; Ana Gracanin; Ruben van Boxtel; John Wongvipat; Catherine M Dowling; Dong Gao; Harry Begthel; Norman Sachs; Robert G J Vries; Edwin Cuppen; Yu Chen; Charles L Sawyers; Hans C Clevers
Journal:  Cell       Date:  2014-09-04       Impact factor: 41.582

7.  Prospective derivation of a living organoid biobank of colorectal cancer patients.

Authors:  Marc van de Wetering; Hayley E Francies; Joshua M Francis; Gergana Bounova; Francesco Iorio; Apollo Pronk; Winan van Houdt; Joost van Gorp; Amaro Taylor-Weiner; Lennart Kester; Anne McLaren-Douglas; Joyce Blokker; Sridevi Jaksani; Sina Bartfeld; Richard Volckman; Peter van Sluis; Vivian S W Li; Sara Seepo; Chandra Sekhar Pedamallu; Kristian Cibulskis; Scott L Carter; Aaron McKenna; Michael S Lawrence; Lee Lichtenstein; Chip Stewart; Jan Koster; Rogier Versteeg; Alexander van Oudenaarden; Julio Saez-Rodriguez; Robert G J Vries; Gad Getz; Lodewyk Wessels; Michael R Stratton; Ultan McDermott; Matthew Meyerson; Mathew J Garnett; Hans Clevers
Journal:  Cell       Date:  2015-05-07       Impact factor: 41.582

8.  Integrative clinical genomics of advanced prostate cancer.

Authors:  Dan Robinson; Eliezer M Van Allen; Yi-Mi Wu; Nikolaus Schultz; Robert J Lonigro; Juan-Miguel Mosquera; Bruce Montgomery; Mary-Ellen Taplin; Colin C Pritchard; Gerhardt Attard; Himisha Beltran; Wassim Abida; Robert K Bradley; Jake Vinson; Xuhong Cao; Pankaj Vats; Lakshmi P Kunju; Maha Hussain; Felix Y Feng; Scott A Tomlins; Kathleen A Cooney; David C Smith; Christine Brennan; Javed Siddiqui; Rohit Mehra; Yu Chen; Dana E Rathkopf; Michael J Morris; Stephen B Solomon; Jeremy C Durack; Victor E Reuter; Anuradha Gopalan; Jianjiong Gao; Massimo Loda; Rosina T Lis; Michaela Bowden; Stephen P Balk; Glenn Gaviola; Carrie Sougnez; Manaswi Gupta; Evan Y Yu; Elahe A Mostaghel; Heather H Cheng; Hyojeong Mulcahy; Lawrence D True; Stephen R Plymate; Heidi Dvinge; Roberta Ferraldeschi; Penny Flohr; Susana Miranda; Zafeiris Zafeiriou; Nina Tunariu; Joaquin Mateo; Raquel Perez-Lopez; Francesca Demichelis; Brian D Robinson; Marc Schiffman; David M Nanus; Scott T Tagawa; Alexandros Sigaras; Kenneth W Eng; Olivier Elemento; Andrea Sboner; Elisabeth I Heath; Howard I Scher; Kenneth J Pienta; Philip Kantoff; Johann S de Bono; Mark A Rubin; Peter S Nelson; Levi A Garraway; Charles L Sawyers; Arul M Chinnaiyan
Journal:  Cell       Date:  2015-05-21       Impact factor: 41.582

9.  RNA-programmed genome editing in human cells.

Authors:  Martin Jinek; Alexandra East; Aaron Cheng; Steven Lin; Enbo Ma; Jennifer Doudna
Journal:  Elife       Date:  2013-01-29       Impact factor: 8.140

10.  GRHL2 coordinates regeneration of a polarized mucociliary epithelium from basal stem cells.

Authors:  Xia Gao; Aman S Bali; Scott H Randell; Brigid L M Hogan
Journal:  J Cell Biol       Date:  2015-11-02       Impact factor: 10.539
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  3 in total

Review 1.  CRISPR in cancer biology and therapy.

Authors:  Alyna Katti; Bianca J Diaz; Christina M Caragine; Neville E Sanjana; Lukas E Dow
Journal:  Nat Rev Cancer       Date:  2022-02-22       Impact factor: 60.716

Review 2.  Engineering Prostate Cancer from Induced Pluripotent Stem Cells-New Opportunities to Develop Preclinical Tools in Prostate and Prostate Cancer Studies.

Authors:  Anastasia C Hepburn; C H Cole Sims; Adriana Buskin; Rakesh Heer
Journal:  Int J Mol Sci       Date:  2020-01-30       Impact factor: 5.923

3.  Rapid interrogation of cancer cell of origin through CRISPR editing.

Authors:  Weiran Feng; Zhen Cao; Pei Xin Lim; Huiyong Zhao; Hanzhi Luo; Ninghui Mao; Young Sun Lee; Aura Agudelo Rivera; Danielle Choi; Chao Wu; Teng Han; Rodrigo Romero; Elisa de Stanchina; Brett S Carver; Qiao Wang; Maria Jasin; Charles L Sawyers
Journal:  Proc Natl Acad Sci U S A       Date:  2021-08-10       Impact factor: 11.205
  3 in total

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