Fouzia Radouani1, Loubna El Yazouli2, Zakaria Elyazghi3, Hicham Hejaji4, Aziz Aroussi Alami5, Naima Elmdaghri6. 1. Research Department, Chlamydiae and Mycoplasma Laboratory, Institut Pasteur du Maroc, 20360, Casablanca, Morocco. Electronic address: radouani@hotmail.com. 2. Research Department, Chlamydiae and Mycoplasma Laboratory, Institut Pasteur du Maroc, 20360, Casablanca, Morocco. Electronic address: l_elyazouli@hotmail.fr. 3. Research Department, Chlamydiae and Mycoplasma Laboratory, Institut Pasteur du Maroc, 20360, Casablanca, Morocco. Electronic address: elyazghi.zakaria@gmail.com. 4. Cardiovascular Surgery Department, CHU Ibn Rochd, 20360, Casablanca, Morocco. Electronic address: hichamhejaji@gmail.com. 5. Cardiovascular Surgery Department, CHU Ibn Rochd, 20360, Casablanca, Morocco. Electronic address: a3alami@hotmail.com. 6. Research Department, Chlamydiae and Mycoplasma Laboratory, Institut Pasteur du Maroc, 20360, Casablanca, Morocco. Electronic address: naima.elmdaghri@yahoo.fr.
Abstract
BACKGROUND: Chlamydia pneumoniae is a pathogen associated with human respiratory tract infection, its viable presence in atherosclerotic plaques is also assumed to play significant function in cardiac diseases. Our study's main objective is to evaluate Chlamydia pneumoniae sero-prevalence in Moroccan patients with cardiovascular diseases using and comparing two serological methods. METHODS: Two hundred eighteen patients were enrolled; serums were tested by microimmunofluorescence to explore the sero-prevalence. Simultaneously 74 serums were analyzed by both immunoblot and micro-immunofluorescence to evaluate recombinant proteins diagnosis value. RESULTS: MIF results revealed 81% male and 84.5% female positive cases. The comparative study among 74 patients showed 78% men and 89% women positive cases by immunoblot, whereas MIF showed respectively 80% and 72%, a significant concordance between these methods was revealed. However, this comparison showed also two types of discrepancies, which may be related to difficulties in antigens detection by micro-immunofluorescence resulting from their structure complexity, or the antibodies reactivity with species' common antigens. CONCLUSIONS: The study revealed a high sero-prevalence of Chlamydia pneumoniae in the studied population, a big interest of recombinant protein was also revealed in the diagnosis accuracy. We suggest therefore using immunoblot for diagnosis confirmation because it provides additional useful information.
BACKGROUND: Chlamydia pneumoniae is a pathogen associated with humanrespiratory tract infection, its viable presence in atherosclerotic plaques is also assumed to play significant function in cardiac diseases. Our study's main objective is to evaluate Chlamydia pneumoniae sero-prevalence in Moroccan patients with cardiovascular diseases using and comparing two serological methods. METHODS: Two hundred eighteen patients were enrolled; serums were tested by microimmunofluorescence to explore the sero-prevalence. Simultaneously 74 serums were analyzed by both immunoblot and micro-immunofluorescence to evaluate recombinant proteins diagnosis value. RESULTS:MIF results revealed 81% male and 84.5% female positive cases. The comparative study among 74 patients showed 78% men and 89% women positive cases by immunoblot, whereas MIF showed respectively 80% and 72%, a significant concordance between these methods was revealed. However, this comparison showed also two types of discrepancies, which may be related to difficulties in antigens detection by micro-immunofluorescence resulting from their structure complexity, or the antibodies reactivity with species' common antigens. CONCLUSIONS: The study revealed a high sero-prevalence of Chlamydia pneumoniae in the studied population, a big interest of recombinant protein was also revealed in the diagnosis accuracy. We suggest therefore using immunoblot for diagnosis confirmation because it provides additional useful information.