| Literature DB >> 30539569 |
Jehan Alam1,2, Sumin Jeon1, Youngnim Choi3.
Abstract
Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.Entities:
Keywords: Aquaporin 5; Autoantibodies; Cell-based assay; Immunofluorescence cytochemistry; Plasma membrane
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Year: 2019 PMID： 30539569 DOI： 10.1007/978-1-4939-8949-2_6
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745