M Dalledone1, A S Cunha2, L A Ramazzotto2,3, G D Pecharki4, P Nelson-Filho2, R Scariot1, P C Trevilatto5, A R Vieira6, Erika C Küchler7,8, João A Brancher9. 1. School of Health and Biological Sciences, Universidade Positivo, Rua Professor Pedro Viriato Parigot de Souza, 5300, Campo Comprido, Curitiba, Paraná, 80740-050, Brazil. 2. Department of Pediatric Dentistry - School of Dentistry of Ribeirão Preto, University of São Paulo, USP, Avenida do Café s/n, Monte Alegre, Ribeirão Preto, São Paulo, 14040-904, Brazil. 3. Biotechnology Graduation, Federal University of São Carlos, São Carlos, Brazil. 4. Department of Community Health, Federal University of Parana, Curitiba, Brazil. 5. School of Life Sciences, Pontifícia Universidade Católica do Paraná, Curitiba, Brazil. 6. Department of Oral Biology, School of Dental Medicine, University of Pittsburgh, Pittsburgh, PA, 15260, USA. 7. School of Health and Biological Sciences, Universidade Positivo, Rua Professor Pedro Viriato Parigot de Souza, 5300, Campo Comprido, Curitiba, Paraná, 80740-050, Brazil. erikacalvano@gmail.com. 8. Department of Pediatric Dentistry - School of Dentistry of Ribeirão Preto, University of São Paulo, USP, Avenida do Café s/n, Monte Alegre, Ribeirão Preto, São Paulo, 14040-904, Brazil. erikacalvano@gmail.com. 9. School of Health and Biological Sciences, Universidade Positivo, Rua Professor Pedro Viriato Parigot de Souza, 5300, Campo Comprido, Curitiba, Paraná, 80740-050, Brazil. brancher.a@gmail.com.
Abstract
OBJECTIVES: The aim of this study was investigate the association between genetic polymorphisms in ESR1, ESR2, and ESRRB and dental fluorosis (DF) in a well-characterized sample of children from Curitiba, Brazil. MATERIAL AND METHODS: From a representative sample of 538 children, 12-year-old were evaluated. DF was assessed in erupted permanent teeth by the Dean's index modified. Fourteen polymorphisms were selected in intronic and intergenic regions of ESR1, ESR2, and ESRRB and genotyped in genomic DNA source from saliva using TaqMan chemistry and end-point analysis. Allele and genotype distributions between DF and DF free groups were analyzed using the Epi Info 7.2. Chi-square or Fisher's exact tests at a level of significance of 5% and odds ratios calculations with 95% confidence intervals were used to determine the statistical associations. RESULTS: Among 538 children, 147 were DF and 391 were DF free. Genotype distribution for the polymorphism rs12154178 in ESR1 was different between the two groups (p = 0.037; OR = 0.91; CI = 0.67-1.22). The dominant model analysis (AA+AC vs. CC) demonstrated that CC is a protective factor for DF (p = 0.038; OR = 0.51, 0.27-0.97 95% CI). We did not find differences in frequency distributions in the other evaluated polymorphisms. CONCLUSION: This study provides evidence that ESR1 is associated with DF. CLINICAL RELEVANCE: Dental fluorosis is an important condition that affects the mineralized tissues of the teeth. In severe cases, the treatment takes time and is extremely costly. This research provides evidences that there are genetic factors involved in dental fluorosis and will help professionals to plan more precise strategies to reduce dental fluorosis occurrence.
OBJECTIVES: The aim of this study was investigate the association between genetic polymorphisms in ESR1, ESR2, and ESRRB and dental fluorosis (DF) in a well-characterized sample of children from Curitiba, Brazil. MATERIAL AND METHODS: From a representative sample of 538 children, 12-year-old were evaluated. DF was assessed in erupted permanent teeth by the Dean's index modified. Fourteen polymorphisms were selected in intronic and intergenic regions of ESR1, ESR2, and ESRRB and genotyped in genomic DNA source from saliva using TaqMan chemistry and end-point analysis. Allele and genotype distributions between DF and DF free groups were analyzed using the Epi Info 7.2. Chi-square or Fisher's exact tests at a level of significance of 5% and odds ratios calculations with 95% confidence intervals were used to determine the statistical associations. RESULTS: Among 538 children, 147 were DF and 391 were DF free. Genotype distribution for the polymorphism rs12154178 in ESR1 was different between the two groups (p = 0.037; OR = 0.91; CI = 0.67-1.22). The dominant model analysis (AA+AC vs. CC) demonstrated that CC is a protective factor for DF (p = 0.038; OR = 0.51, 0.27-0.97 95% CI). We did not find differences in frequency distributions in the other evaluated polymorphisms. CONCLUSION: This study provides evidence that ESR1 is associated with DF. CLINICAL RELEVANCE: Dental fluorosis is an important condition that affects the mineralized tissues of the teeth. In severe cases, the treatment takes time and is extremely costly. This research provides evidences that there are genetic factors involved in dental fluorosis and will help professionals to plan more precise strategies to reduce dental fluorosis occurrence.
Authors: Trina Mylena García-Escobar; Iván Valdivia-Gandur; Wilson Astudillo-Rozas; Oscar Aceituno-Antezana; Balasubbaiah Yamadala; Vicente Lozano de Luaces; Eduardo Chimenos-Küstner; María Cristina Manzanares-Céspedes Journal: Int J Environ Res Public Health Date: 2022-09-08 Impact factor: 4.614