Virus titre determines the efficiency of Pentalonia nigronervosa (Aphididae: Hemiptera) to transmit banana bunchy top virus.

Banana bunchy top disease (BBTD) caused by banana bunchy top virus (BBTV) is one of the most serious viral diseases of banana and plantains. BBTV is transmitted by Pentalonia nigronervosa (Hemiptera, Aphididae) in a persistent circulative manner. Better knowledge of vector-virus-host relationship and the mechanism of transmission is essential for developing an effective control strategy. In this study, the viral copies in single to group of aphids with different acquisition access period (AAP) were quantified using SYBR green-based quantitative polymerase chain reaction (qPCR). The result indicated that a single aphid was able to acquire 861.04 copies of the virus after 24 h of AAP from the infected banana plant and transmitted the virus to 16.6% tissue culture plants, whereas 50 viruliferous aphids (15,066.94 viral copies) were necessary to achieve 100% transmission in a shortest time of 21.6 days. The number of viral copies acquired by the aphids were gradually increased with increased AAP. Hundred percent transmission was observed with 20 aphids in 48 h of AAP or 30-50 aphids in 24 h of AAP. The inoculated plants expressed typical bunchy top symptoms quickly when higher number of aphids (30 and above) were used with 24 h of AAP. Further, we report that the tissue culture banana plants are highly prone or vulnerable to BBTV infection compared to sucker grown plants. We conclude that higher the number of viral copies in the vector, higher the percent transmission and quicker the symptom expression and the results will contribute to a better understanding of vector-BBTV interactions and useful for epidemiological studies.