Berna Ayan1, Nilay Yuksel2, Ahmet Carhan3, Berrak Gumuşkaya Ocal4, Emine Akcay5, Nurullah Cagil5, Mehmet Dogan Asik6. 1. Samsun Carsamba State Hospital, Department of Ophthalmology, Samsun, Turkey. 2. Ankara Ataturk Education and Research Hospital, Department of Ophthalmology, Ankara, Turkey. Electronic address: ozturk.nilay@gmail.com. 3. Yildirim Beyazit University, Ankara Ataturk Education and Research Hospital, Department of Medical Biology, Ankara, Turkey. 4. Yildirim Beyazit University, Ankara Ataturk Education and Research Hospital, Department of Pathology, Ankara, Turkey. 5. Yildirim Beyazit University, Ankara Ataturk Education and Research Hospital, Department of Ophthalmology, Ankara, Turkey. 6. Hacettepe University, Bioengineering, Ankara, Turkey.
Abstract
PURPOSE: To evaluate the role of sex steroid hormone receptors in corneal epithelium in etiopathogenesis of keratoconus (KC). METHODS: Thirty patients with KC who were planned for corneal collagen-crosslinking and 20 patients who were planned for excimer laser for refractive errors included in this study. Corneal epitheliums were curated mechanically during surgeries. Right eyes were evaluated immunohistochemically and left eyes were evaluated by quantitative polymerase chain reaction (qPCR) to investigate estrogenα, estrogenβ, progesterone and androgen receptors. RESULTS: Immunohistochemically, staining for progesterone and androgen receptors did not significantly differ between KC and control groups (p > 0.05). None of the cases had staining for estrogenα and estrogenβ receptors. qPCR showed that mRNA expressions of estrogenα and androgen receptors were significantly higher in the KC group (p < 0.001). CONCLUSION: A significantly higher rate of estrogenα and androgen receptor expressions in corneal epithelium from patients with KC through qPCR supports a possible relation between KC and sex steroid hormones.
PURPOSE: To evaluate the role of sex steroid hormone receptors in corneal epithelium in etiopathogenesis of keratoconus (KC). METHODS: Thirty patients with KC who were planned for corneal collagen-crosslinking and 20 patients who were planned for excimer laser for refractive errors included in this study. Corneal epitheliums were curated mechanically during surgeries. Right eyes were evaluated immunohistochemically and left eyes were evaluated by quantitative polymerase chain reaction (qPCR) to investigate estrogenα, estrogenβ, progesterone and androgen receptors. RESULTS: Immunohistochemically, staining for progesterone and androgen receptors did not significantly differ between KC and control groups (p > 0.05). None of the cases had staining for estrogenα and estrogenβ receptors. qPCR showed that mRNA expressions of estrogenα and androgen receptors were significantly higher in the KC group (p < 0.001). CONCLUSION: A significantly higher rate of estrogenα and androgen receptor expressions in corneal epithelium from patients with KC through qPCR supports a possible relation between KC and sex steroid hormones.
Authors: Dimitrios Karamichos; Paulina Escandon; Brenda Vasini; Sarah E Nicholas; Lyly Van; Deanna H Dang; Rebecca L Cunningham; Kamran M Riaz Journal: Prog Retin Eye Res Date: 2021-11-02 Impact factor: 19.704
Authors: Paulina Escandon; Sarah E Nicholas; Rebecca L Cunningham; David A Murphy; Kamran M Riaz; Dimitrios Karamichos Journal: Int J Mol Sci Date: 2022-01-14 Impact factor: 5.923