Izael S Costa1, Amanda F Medeiros2, Fabiana M C Carvalho2, Vanessa C O Lima2, Raphael P Serquiz2, Alexandre C Serquiz3, Vivian N Silbiger1,4, Raul H Bortolin4, Bruna L L Maciel1, Elizeu A Santos1, Ana H A Morais5,6. 1. Nutrition Postgraduate Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil. 2. Biochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil. 3. Nutrition Curse, University Center of Rio Grande do Norte, Natal, Brazil. 4. Department of Clinical and Toxicological Analysis, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil. 5. Nutrition Postgraduate Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal, Brazil, aharaujomorais@gmail.com. 6. Biochemistry Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal, Brazil, aharaujomorais@gmail.com.
Abstract
OBJECTIVE: This study evaluated the effect of a protein, the isolated Trypsin Inhibitor (TTI) from Tamarindus indica L. seed, as a CCK secretagogue and its action upon food intake and leptin in obese Wistar rats. METHODS: Three groups of obese rats were fed 10 days one of the following diets: Standard diet (Labina®) + water; High Glycemic Index and Load (HGLI) diet + water or HGLI diet + TTI. Lean animals were fed the standard diet for the 10 days. Food intake, zoometric measurements, plasma CCK, plasma leptin, relative mRNA expression of intestinal CCK-related genes, and expression of the ob gene in subcutaneous adipose tissue were assessed. RESULTS: TTI decreased food intake but did not increase plasma CCK in obese animals. On the other hand, TTI treatment decreased CCK-1R gene expression in obese animals compared with the obese group with no treatment (p = 0.027). Obese animals treated with TTI presented lower plasma leptin than the non-treated obese animals. CONCLUSION: We suggest that TTI by decreasing plasma leptin may improve CCK action, regardless of its increase in plasma from obese rats, since food intake was lowest.
OBJECTIVE: This study evaluated the effect of a protein, the isolated Trypsin Inhibitor (TTI) from Tamarindus indica L. seed, as a CCK secretagogue and its action upon food intake and leptin in obeseWistar rats. METHODS: Three groups of obeserats were fed 10 days one of the following diets: Standard diet (Labina®) + water; High Glycemic Index and Load (HGLI) diet + water or HGLI diet + TTI. Lean animals were fed the standard diet for the 10 days. Food intake, zoometric measurements, plasma CCK, plasma leptin, relative mRNA expression of intestinal CCK-related genes, and expression of the ob gene in subcutaneous adipose tissue were assessed. RESULTS: TTI decreased food intake but did not increase plasma CCK in obese animals. On the other hand, TTI treatment decreased CCK-1R gene expression in obese animals compared with the obese group with no treatment (p = 0.027). Obese animals treated with TTI presented lower plasma leptin than the non-treated obese animals. CONCLUSION: We suggest that TTI by decreasing plasma leptin may improve CCK action, regardless of its increase in plasma from obeserats, since food intake was lowest.
Authors: M Buyse; M L Ovesjö; H Goïot; S Guilmeau; G Péranzi; L Moizo; F Walker; M J Lewin; B Meister; A Bado Journal: Eur J Neurosci Date: 2001-07 Impact factor: 3.386
Authors: Ayman I Sayegh; Martha C Washington; Ruth E Johnson; Tanisha Johnson-Rouse; Corren Freeman; Anna Harrison; Jennifer Lucas; Mandy Shelby; Brittley Fisher; William Willis; Joseph J Reeve Journal: Horm Behav Date: 2014-12-02 Impact factor: 3.587
Authors: Lídia L R Matias; Rafael O A Costa; Thaís S Passos; Jaluza L C Queiroz; Alexandre C Serquiz; Bruna L L Maciel; Pedro P A Santos; Christina S Camillo; Catarina Gonçalves; Isabel R Amado; Lorenzo Pastrana; Ana H A Morais Journal: Nutrients Date: 2019-11-14 Impact factor: 5.717
Authors: Amanda Fernandes de Medeiros; Beatriz Blenda Pinheiro de Souza; Lucas Pinheiro Coutinho; Aline Melro Murad; Paula Ivani Medeiros Dos Santos; Norberto de Kássio Vieira Monteiro; Elizeu Antunes Dos Santos; Bruna Leal Lima Maciel; Ana Heloneida de Araújo Morais Journal: J Enzyme Inhib Med Chem Date: 2021-12 Impact factor: 5.051
Authors: Ana H de A Morais; Amanda F de Medeiros; Isaiane Medeiros; Vanessa C O de Lima; Anna B S Luz; Bruna L L Maciel; Thaís S Passos Journal: Drug Target Insights Date: 2021-04-02