Hao Zhang1,2,3, Yue Li4, Yueping Chen1,5, Lili Zhang1, Tian Wang6. 1. College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang Road, Nanjing, 210095, Jiangsu, People's Republic of China. 2. Postdoctoral Research Station of Clinical Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, No. 1, Weigang Road, Nanjing, 210095, Jiangsu, People's Republic of China. 3. Shanghai Key Laboratory of Veterinary Biotechnology, Shanghai, 200240, People's Republic of China. 4. Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, 50 Zhongling Street, Nanjing, 210014, Jiangsu, People's Republic of China. 5. Postdoctoral Research Station of Food Science and Engineering, College of Food Science and Technology, Nanjing Agricultural University, No. 1, Weigang Road, Nanjing, 210095, People's Republic of China. 6. College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang Road, Nanjing, 210095, Jiangsu, People's Republic of China. tianwangnjau@163.com.
Abstract
PURPOSE: Intrauterine growth retardation (IUGR) is detrimental to the intestinal development of neonates, yet satisfactory treatment strategies remain limited. This study was, therefore, conducted using neonatal piglets as a model to investigate the potential of N-acetylcysteine (NAC) to alleviate intestinal damage caused by IUGR. METHODS: Seven normal birth weight (NBW) and fourteen IUGR neonatal male piglets were selected and then fed a basal milk diet (NBW-CON and IUGR-CON groups) or a basal milk diet supplemented with 1.2 g NAC per kg of diet (IUGR-NAC group) from 7 to 21 days of age (n = 7). Parameters associated with the severity of intestinal injury, villus morphology and ultrastructural structure, redox status, and mitochondrial function were analyzed. RESULTS: Compared with the NBW-CON piglets, the IUGR-CON piglets exhibited decreased villus height and greater numbers of apoptotic cells in jejunum, along with the increases in malondialdehyde and protein carbonyl concentrations and a decreased adenosine triphosphate (ATP) content. Treatment with NAC significantly increased jejunal superoxide dismutase activity, reduced glutathione: oxidized glutathione ratio, and the mRNA abundance of nuclear respiratory factor 2, heme oxygenase 1, and superoxide dismutase 2 in the IUGR-NAC piglets compared with the IUGR-CON piglets. In addition, NAC improved the efficiency of mitochondrial oxidative metabolism and ATP generation, ameliorated mitochondrial swelling, and inhibited the overproduction of mitochondrial superoxide anion in the jejunal mucosa. CONCLUSIONS: Dietary supplementation of NAC shows promise for attenuating the early intestinal injury of young piglets with IUGR, probably through its antioxidant action to restore redox status and mitochondrial function.
PURPOSE:Intrauterine growth retardation (IUGR) is detrimental to the intestinal development of neonates, yet satisfactory treatment strategies remain limited. This study was, therefore, conducted using neonatal piglets as a model to investigate the potential of N-acetylcysteine (NAC) to alleviate intestinal damage caused by IUGR. METHODS: Seven normal birth weight (NBW) and fourteen IUGR neonatal male piglets were selected and then fed a basal milk diet (NBW-CON and IUGR-CON groups) or a basal milk diet supplemented with 1.2 g NAC per kg of diet (IUGR-NAC group) from 7 to 21 days of age (n = 7). Parameters associated with the severity of intestinal injury, villus morphology and ultrastructural structure, redox status, and mitochondrial function were analyzed. RESULTS: Compared with the NBW-CON piglets, the IUGR-CON piglets exhibited decreased villus height and greater numbers of apoptotic cells in jejunum, along with the increases in malondialdehyde and protein carbonyl concentrations and a decreased adenosine triphosphate (ATP) content. Treatment with NAC significantly increased jejunal superoxide dismutase activity, reduced glutathione: oxidized glutathione ratio, and the mRNA abundance of nuclear respiratory factor 2, heme oxygenase 1, and superoxide dismutase 2 in the IUGR-NAC piglets compared with the IUGR-CON piglets. In addition, NAC improved the efficiency of mitochondrial oxidative metabolism and ATP generation, ameliorated mitochondrial swelling, and inhibited the overproduction of mitochondrial superoxide anion in the jejunal mucosa. CONCLUSIONS: Dietary supplementation of NAC shows promise for attenuating the early intestinal injury of young piglets with IUGR, probably through its antioxidant action to restore redox status and mitochondrial function.
Authors: C Mandò; S Tabano; P Colapietro; P Pileri; F Colleoni; L Avagliano; P Doi; G Bulfamante; M Miozzo; I Cetin Journal: Placenta Date: 2010-10-30 Impact factor: 3.481
Authors: Hong-wa Yung; Stefania Calabrese; Debby Hynx; Brian A Hemmings; Irene Cetin; D Stephen Charnock-Jones; Graham J Burton Journal: Am J Pathol Date: 2008-06-26 Impact factor: 4.307
Authors: Miriam Ayuso; Laura Buyssens; Marina Stroe; Allan Valenzuela; Karel Allegaert; Anne Smits; Pieter Annaert; Antonius Mulder; Sebastien Carpentier; Chris Van Ginneken; Steven Van Cruchten Journal: Pharmaceutics Date: 2020-12-30 Impact factor: 6.321