Zhengfei Wang1, Dan Tang2, Linxia Sun2, Xueling Shi2, Ruobing Liu2, Huayun Guo2, Boping Tang3. 1. Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224001, Jiangsu Province, China. wangzf@yctu.edu.cn. 2. Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224001, Jiangsu Province, China. 3. Jiangsu Key Laboratory for Bioresources of Saline Soils, Jiangsu Synthetic Innovation Center for Coastal Bio-agriculture, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, School of Ocean and Biological Engineering, Yancheng Teachers University, Yancheng, 224001, Jiangsu Province, China. boptang@163.com.
Abstract
BACKGROUND: The mudflat crab Helice tientsinensis is one of the most commercially valuable species for crabmeat production due to its delicious taste. These crabs are mainly found in coastal wetland where they are seriously threatened by toxic heavy metal pollution. In crustaceans, the hepatopancreas is an important organ for detoxification, and metal toxic substances can be converted to non-toxic or less toxic compounds in this organ. OBJECTIVE: To develop a better understanding of the molecular response of H. tientsinensis to the toxic metal cadmium (Cd) and provide a molecular basis for the toxic metal tolerance of H. tientsinensis. METHODS: In this study, we performed comparative hepatopancreas transcriptome analysis between H. tientsinensis unexposed (as control) and exposed to the toxic metal Cd for 48 h. RESULTS: We identified 1089 Cd stress significantly-upregulated and 1560 Cd stress significantly-downregulated unigenes. Functional categorization and annotation of these differentially-expressed genes (DEGs) demonstrated that the response to Cd stress in the hepatopancreas of H. tientsinensis mainly involves "antioxidant activity", "detoxification", "toxin degradation activity" and "immune system process". In addition, five genes (ABCC1, NDUFAF5, ASTL, DES1, CYP27A) were identified as possible major targets for toxic metal tolerance. CONCLUSION: This is the first time reporting that the response of H. tientsinensis to Cd exposure at the transcriptome level, and it lays the foundation for understanding the molecular mechanisms of the response of H. tientsinensis to environmental toxic metal stress.
BACKGROUND: The mudflat crab Helice tientsinensis is one of the most commercially valuable species for crabmeat production due to its delicious taste. These crabs are mainly found in coastal wetland where they are seriously threatened by toxic heavy metal pollution. In crustaceans, the hepatopancreas is an important organ for detoxification, and metal toxic substances can be converted to non-toxic or less toxic compounds in this organ. OBJECTIVE: To develop a better understanding of the molecular response of H. tientsinensis to the toxic metalcadmium (Cd) and provide a molecular basis for the toxic metal tolerance of H. tientsinensis. METHODS: In this study, we performed comparative hepatopancreas transcriptome analysis between H. tientsinensis unexposed (as control) and exposed to the toxic metalCd for 48 h. RESULTS: We identified 1089 Cd stress significantly-upregulated and 1560 Cd stress significantly-downregulated unigenes. Functional categorization and annotation of these differentially-expressed genes (DEGs) demonstrated that the response to Cd stress in the hepatopancreas of H. tientsinensis mainly involves "antioxidant activity", "detoxification", "toxin degradation activity" and "immune system process". In addition, five genes (ABCC1, NDUFAF5, ASTL, DES1, CYP27A) were identified as possible major targets for toxic metal tolerance. CONCLUSION: This is the first time reporting that the response of H. tientsinensis to Cd exposure at the transcriptome level, and it lays the foundation for understanding the molecular mechanisms of the response of H. tientsinensis to environmental toxic metal stress.
Entities:
Keywords:
Cadmium stress; Detoxification; Helice tientsinensis; Toxic metal tolerance; Transcriptome
Authors: Eduardo Alves de Almeida; Afonso Celso Dias Bainy; Ana Paula de Melo Loureiro; Glaucia Regina Martinez; Sayuri Miyamoto; Janice Onuki; Lívea Fujita Barbosa; Camila Carrião Machado Garcia; Fernanda Manso Prado; Graziella Eliza Ronsein; Carlos Alexandre Sigolo; Cláudia Barbosa Brochini; Ana Maria Gracioso Martins; Marisa Helena Gennari de Medeiros; Paolo Di Mascio Journal: Comp Biochem Physiol A Mol Integr Physiol Date: 2006-04-19 Impact factor: 2.320
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