BACKGROUND: Obesity is an independent risk factor for increased influenza mortality and is associated with impaired memory T-cell response, resulting in increased risk of infection. In this study, we investigated if weight loss would restore memory T-cell response to influenza. METHODS: Male C57BL/6J mice were fed either low-fat or high-fat diet to induce obesity. Once obesity was established, all mice received primary infection with influenza X-31. Following a recovery period, we switched half of the obese group to a low-fat diet to induce weight loss. Fifteen weeks after diet switch, all mice were given a secondary infection with influenza PR8, and memory T-cell function and T-cell metabolism were measured. RESULTS: Following secondary influenza infection, memory T-cell subsets in the lungs of obese mice were decreased compared to lean mice. At the same time, T cells from obese mice were found to have altered cellular metabolism, largely characterized by an increase in oxygen consumption. Neither impaired memory T-cell response nor altered T-cell metabolism was reversed with weight loss. CONCLUSION: Obesity-associated changes in T-cell metabolism are associated with impaired T-cell response to influenza, and are not reversed with weight loss.
BACKGROUND:Obesity is an independent risk factor for increased influenza mortality and is associated with impaired memory T-cell response, resulting in increased risk of infection. In this study, we investigated if weight loss would restore memory T-cell response to influenza. METHODS: Male C57BL/6J mice were fed either low-fat or high-fat diet to induce obesity. Once obesity was established, all mice received primary infection with influenza X-31. Following a recovery period, we switched half of the obese group to a low-fat diet to induce weight loss. Fifteen weeks after diet switch, all mice were given a secondary infection with influenza PR8, and memory T-cell function and T-cell metabolism were measured. RESULTS: Following secondary influenza infection, memory T-cell subsets in the lungs of obesemice were decreased compared to lean mice. At the same time, T cells from obesemice were found to have altered cellular metabolism, largely characterized by an increase in oxygen consumption. Neither impaired memory T-cell response nor altered T-cell metabolism was reversed with weight loss. CONCLUSION:Obesity-associated changes in T-cell metabolism are associated with impaired T-cell response to influenza, and are not reversed with weight loss.
Authors: Erika L Pearce; Matthew C Walsh; Pedro J Cejas; Gretchen M Harms; Hao Shen; Li-San Wang; Russell G Jones; Yongwon Choi Journal: Nature Date: 2009-06-03 Impact factor: 49.962
Authors: P A Sheridan; H A Paich; J Handy; E A Karlsson; M G Hudgens; A B Sammon; L A Holland; S Weir; T L Noah; M A Beck Journal: Int J Obes (Lond) Date: 2011-10-25 Impact factor: 5.095
Authors: S D Neidich; W D Green; J Rebeles; E A Karlsson; S Schultz-Cherry; T L Noah; S Chakladar; M G Hudgens; S S Weir; M A Beck Journal: Int J Obes (Lond) Date: 2017-06-06 Impact factor: 5.095
Authors: Heather A Paich; Patricia A Sheridan; Jean Handy; Erik A Karlsson; Stacey Schultz-Cherry; Michael G Hudgens; Terry L Noah; Samuel S Weir; Melinda A Beck Journal: Obesity (Silver Spring) Date: 2013-05-30 Impact factor: 5.002