| Literature DB >> 3053245 |
R Jerala1, M Trstenjak, B Lenarcic, V Turk.
Abstract
A gene coding for human stefin B was synthesized by the solid-phase phosphite method and cloned in the pUC8 cloning vector. The insert with the verified DNA sequence was subcloned into two expression vectors and expressed in E. coli as a fusion protein with beta-galactosidase and as a native protein. The CNBr cleaved fusion protein and the native recombinant stefin B were inhibitory to papain and reacted with antibodies against human stefin B.Entities:
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Year: 1988 PMID： 3053245 DOI： 10.1016/0014-5793(88)80541-6
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124