Literature DB >> 3053166

Sensitive and high resolution in situ hybridization to human chromosomes using biotin labelled probes: assignment of the human thymocyte CD1 antigen genes to chromosome 1.

D G Albertson1, R Fishpool, P Sherrington, E Nacheva, C Milstein.   

Abstract

A method for in situ hybridization originally developed for mapping genes in the nematode, Caenorhabditis elegans has been adapted for high resolution cytological mapping of genes in the human. The probe DNAs are labelled by incorporation of biotin dUTP and the site of hybridization detected by immunofluorescence. For the accurate assignment of the hybridization signal to chromosome bands, visualized by staining with Hoechst 33258, a heterologous ribosomal DNA probe is also included in the hybridization reaction. These rDNA signals are used as fiducial markers when aligning the two fluorescent images. We demonstrate the method by assignment of the human thymocyte CD1 antigen genes to human chromosome 1q22-23.

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Year:  1988        PMID： 3053166      PMCID： PMC457071          DOI： 10.1002/j.1460-2075.1988.tb03135.x

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  14 in total

1.  A novel family of human major histocompatibility complex-related genes not mapping to chromosome 6.

Authors:  F Calabi; C Milstein
Journal:  Nature       Date:  1986 Oct 9-15       Impact factor: 49.962

2.  Isolation of CD1 genes: a family of major histocompatibility complex-related differentiation antigens.

Authors:  L H Martin; F Calabi; C Milstein
Journal:  Proc Natl Acad Sci U S A       Date:  1986-12       Impact factor: 11.205

3.  Formation and detection of RNA-DNA hybrid molecules in cytological preparations.

Authors:  J G Gall; M L Pardue
Journal:  Proc Natl Acad Sci U S A       Date:  1969-06       Impact factor: 11.205

4.  Characterization of banding patterns of metaphase-prophase G-banded chromosomes and their use in gene mapping.

Authors:  J J Yunis; J R Sawyer; D W Ball
Journal:  Cytogenet Cell Genet       Date:  1978

5.  Immunological method for mapping genes on Drosophila polytene chromosomes.

Authors:  P R Langer-Safer; M Levine; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

6.  Ribosomal DNA of Caenorhabditis elegans.

Authors:  J G Files; D Hirsh
Journal:  J Mol Biol       Date:  1981-06-25       Impact factor: 5.469

7.  Mid-prophase human chromosomes. The attainment of 2000 bands.

Authors:  J J Yunis
Journal:  Hum Genet       Date:  1981       Impact factor: 4.132

8.  Gene mapping and physical arrangements of human chromatin in transformed, hybrid cells: fluorescent and autoradiographic in situ hybridization compared.

Authors:  A R Mitchell; P Ambros; J R Gosden; J E Morten; D J Porteous
Journal:  Somat Cell Mol Genet       Date:  1986-07

9.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

10.  Localization of the ribosomal genes in Caenorhabditis elegans chromosomes by in situ hybridization using biotin-labeled probes.

Authors:  D G Albertson
Journal:  EMBO J       Date:  1984-06       Impact factor: 11.598
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  26 in total

1.  Genomic anatomy of a premier major histocompatibility complex paralogous region on chromosome 1q21-q22.

Authors:  T Shiina; A Ando; Y Suto; F Kasai; A Shigenari; N Takishima; E Kikkawa; K Iwata; Y Kuwano; Y Kitamura; Y Matsuzawa; K Sano; M Nogami; H Kawata; S Li; Y Fukuzumi; M Yamazaki; H Tashiro; G Tamiya; A Kohda; K Okumura; T Ikemura; E Soeda; N Mizuki; M Kimura; S Bahram; H Inoko
Journal:  Genome Res       Date:  2001-05       Impact factor: 9.043

Review 2.  Molecular biology in medicine.

Authors:  B D Young
Journal:  Postgrad Med J       Date:  1992-04       Impact factor: 2.401

3.  HLA class I homologous transcripts in the human embryonal carcinoma cell line Tera-2.

Authors:  T F Rinke de Wit; L Struyk; S Vloemans; J Glazebrook; J M Boyle; P L Stern; P J van den Elsen
Journal:  Immunogenetics       Date:  1990       Impact factor: 2.846

4.  Bird genes give new insights into the origins of lipid antigen presentation.

Authors:  Steven A Porcelli
Journal:  Proc Natl Acad Sci U S A       Date:  2005-06-06       Impact factor: 11.205

5.  Sensitive detection of RNAs in single cells by flow cytometry.

Authors:  H Yu; L Ernst; M Wagner; A Waggoner
Journal:  Nucleic Acids Res       Date:  1992-01-11       Impact factor: 16.971

6.  Fluorescent in situ hybridization to soybean metaphase chromosomes.

Authors:  M C Griffor; L O Vodkin; R J Singh; T Hymowitz
Journal:  Plant Mol Biol       Date:  1991-07       Impact factor: 4.076

7.  Differential distribution of long and short interspersed element sequences in the mouse genome: chromosome karyotyping by fluorescence in situ hybridization.

Authors:  A L Boyle; S G Ballard; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

8.  Localization of polymorphic DNA probes frequently deleted in lung carcinoma.

Authors:  D G Albertson; P D Sherrington; P H Rabbitts
Journal:  Hum Genet       Date:  1989-09       Impact factor: 4.132

Review 9.  Coevolution of T-cell receptors with MHC and non-MHC ligands.

Authors:  Caitlin D Castro; Adrienne M Luoma; Erin J Adams
Journal:  Immunol Rev       Date:  2015-09       Impact factor: 12.988

10.  Human high-affinity Fc gamma RI (CD64) gene mapped to chromosome 1q21.2-q21.3 by fluorescence in situ hybridization.

Authors:  S Takai; M Kasama; K Yamada; N Kai; N Hirayama; H Namiki; T Taniyama
Journal:  Hum Genet       Date:  1994-01       Impact factor: 4.132
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