Yao Lin1, Dehong Ma2, Songjiao Wen1, Fen Zeng2, Shan Hong3, Lihua Li2, Xiaoman Li4, Xiaodan Wang1, Zhiqiang Ma2, Yue Pan1, Junying Chen1, Juemin Xi1, Lijuan Qiu4, Xiyun Shan5, Qiangming Sun6. 1. Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, PR China; Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR China; Yunnan Key Laboratory of Vector-borne Infectious Disease, Kunming, PR China. 2. Xishuangbanna Dai Autonomous Prefecture People's Hospital, Xishuangbanna, PR China. 3. Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, PR China; Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR China; Kunming Medical University, Kunming, PR China. 4. The Affiliated Children's Hospital of Kunming Medical University, Institute of Pediatric Disease Research, Kunming, PR China. 5. Xishuangbanna Dai Autonomous Prefecture People's Hospital, Xishuangbanna, PR China. Electronic address: sgtrhh@126.com. 6. Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, PR China; Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, PR China; Yunnan Key Laboratory of Vector-borne Infectious Disease, Kunming, PR China. Electronic address: msun08@yahoo.com.
Abstract
BACKGROUND: Xishuangbanna, a border area of China, Burma and Laos, had its first major DENV-1 outbreak in 2017. This study aims to explore the genetic characterization, potential source and evolution of the viruses in outbreak. METHODS: The structural protein C/prM/E genes of viruses isolated from local residents or Burmese travelers were sequenced followed by mutation, phylogenetic, homologous recombination, molecular clock and demographic reconstruction analysis. RESULTS: Phylogenetic analysis revealed that all of the strains were classified as three cluster of DENV-1. Cluster 1, 2 and 3 were most similar to China Guangzhou 2011, China Hubei 2014 and Laos 2008 strain, respectively. Among 236 base mutations, 31 caused nonsynonymous mutations when compared with the DENV-1SS. No homologous recombination signal was discovered. The structural protein of these strains had similar three-dimensional structure. Only site 434 showed differences among five predicted protein binding sites. Molecular clock phylogenetic and demographic reconstruction analysis showed that DENV-1 became highly diversified in 1972 followed by a slightly decreased period until 2017. CONCLUSIONS: Dengue isolated strains show diversification between Burma and China. Amino acid substitution (I440T) may lead to weakened virulence of the epidemic strains. DENV-1 became highly diversified in 1972 followed by a slightly decreased period.
BACKGROUND: Xishuangbanna, a border area of China, Burma and Laos, had its first major DENV-1 outbreak in 2017. This study aims to explore the genetic characterization, potential source and evolution of the viruses in outbreak. METHODS: The structural protein C/prM/E genes of viruses isolated from local residents or Burmese travelers were sequenced followed by mutation, phylogenetic, homologous recombination, molecular clock and demographic reconstruction analysis. RESULTS: Phylogenetic analysis revealed that all of the strains were classified as three cluster of DENV-1. Cluster 1, 2 and 3 were most similar to China Guangzhou 2011, China Hubei 2014 and Laos 2008 strain, respectively. Among 236 base mutations, 31 caused nonsynonymous mutations when compared with the DENV-1SS. No homologous recombination signal was discovered. The structural protein of these strains had similar three-dimensional structure. Only site 434 showed differences among five predicted protein binding sites. Molecular clock phylogenetic and demographic reconstruction analysis showed that DENV-1 became highly diversified in 1972 followed by a slightly decreased period until 2017. CONCLUSIONS: Dengue isolated strains show diversification between Burma and China. Amino acid substitution (I440T) may lead to weakened virulence of the epidemic strains. DENV-1 became highly diversified in 1972 followed by a slightly decreased period.
Authors: Xiaobo Liu; Keke Liu; Yujuan Yue; Haixia Wu; Shu Yang; Yuhong Guo; Dongsheng Ren; Ning Zhao; Jun Yang; Qiyong Liu Journal: Front Public Health Date: 2021-01-18