Shanella Nallaiah1, Valerie Ming Yi Lee1, Helen D Brasch1, Jennifer de Jongh1, Bede van Schaijik1, Reginald Marsh2, Swee T Tan3, Tinte Itinteang1. 1. Gillies McIndoe Research Institute, PO Box 7184, Newtown, Wellington 6242, New Zealand. 2. Gillies McIndoe Research Institute, PO Box 7184, Newtown, Wellington 6242, New Zealand; Auckland University, Private Bag 92019, Victoria St, West Auckland 1142, New Zealand. 3. Gillies McIndoe Research Institute, PO Box 7184, Newtown, Wellington 6242, New Zealand; Wellington Regional Plastic, Maxillofacial & Burns Unit, Hutt Hospital, Private Bag 31709, Lower Hutt, New Zealand. Electronic address: swee.tan@gmri.org.nz.
Abstract
PURPOSE: To investigate the expression of components of the renin-angiotensin system (RAS): pro-renin receptor (PRR), angiotensin converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1) and angiotensin II receptor 2 (ATIIR2) by the cancer stem cell (CSC) subpopulations in moderately differentiated head and neck cutaneous squamous cell carcinoma (MDHNCSCC). METHODOLOGY: 3,3-Diaminobenzidine (DAB) immunohistochemical (IHC) staining for PRR, ACE, ATIIR1 and ATIIR2 was performed on formalin-fixed paraffin-embedded sections of ten MDHNCSCC tissue samples. Immunofluorescence (IF) IHC staining was used to localise components of the RAS. Western blotting (WB) and RT-qPCR were performed on snap-frozen MDHNCSCC tissue samples and MDHNCSCC-derived primary cell lines to investigate protein transcription expression of these proteins, respectively. RESULTS: DAB IHC staining demonstrated the presence of PRR, ACE, ATIIR1 and ATIIR2 in all ten MDHNCSCC tissue samples. IF IHC staining showed expression of PRR and ATIIR2 by the OCT4+ cells, and ACE and ATIIR1 by the SOX2+ cells, within the tumour nests (TNs) and the peritumoural stroma (PTS). PRR, ACE, ATIIR1 and ATIIR2 were expressed by the endothelium of the microvessels within the PTS. WB confirmed protein expression for PRR, ACE and ATIIR1 in MDHNCSCC tissue samples and MDHNCSCC-derived primary cell lines. RT-qPCR showed transcriptional activation of PRR, ACE, ATIIR1 and ATIIR2 in MDHNCSCC tissue samples; and PRR, ACE, ATIIR1 but not ATIIR2, in MDHNCSCC-derived primary cell lines. CONCLUSION: PRR, ACE, ATIIR1 and ATIIR2 are expressed by the CSC subpopulations within the TNs, the PTS, and the endothelium of the microvessels within the PTS, in MDHNCSCC.
PURPOSE: To investigate the expression of components of the renin-angiotensin system (RAS): pro-renin receptor (PRR), angiotensin converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1) and angiotensin II receptor 2 (ATIIR2) by the cancer stem cell (CSC) subpopulations in moderately differentiated head and neck cutaneous squamous cell carcinoma (MDHNCSCC). METHODOLOGY:3,3-Diaminobenzidine (DAB) immunohistochemical (IHC) staining for PRR, ACE, ATIIR1 and ATIIR2 was performed on formalin-fixed paraffin-embedded sections of ten MDHNCSCC tissue samples. Immunofluorescence (IF) IHC staining was used to localise components of the RAS. Western blotting (WB) and RT-qPCR were performed on snap-frozen MDHNCSCC tissue samples and MDHNCSCC-derived primary cell lines to investigate protein transcription expression of these proteins, respectively. RESULTS:DAB IHC staining demonstrated the presence of PRR, ACE, ATIIR1 and ATIIR2 in all ten MDHNCSCC tissue samples. IF IHC staining showed expression of PRR and ATIIR2 by the OCT4+ cells, and ACE and ATIIR1 by the SOX2+ cells, within the tumour nests (TNs) and the peritumoural stroma (PTS). PRR, ACE, ATIIR1 and ATIIR2 were expressed by the endothelium of the microvessels within the PTS. WB confirmed protein expression for PRR, ACE and ATIIR1 in MDHNCSCC tissue samples and MDHNCSCC-derived primary cell lines. RT-qPCR showed transcriptional activation of PRR, ACE, ATIIR1 and ATIIR2 in MDHNCSCC tissue samples; and PRR, ACE, ATIIR1 but not ATIIR2, in MDHNCSCC-derived primary cell lines. CONCLUSION:PRR, ACE, ATIIR1 and ATIIR2 are expressed by the CSC subpopulations within the TNs, the PTS, and the endothelium of the microvessels within the PTS, in MDHNCSCC.
Authors: Therese Featherston; Helen D Brasch; Sam D Siljee; Bede van Schaijik; Josie Patel; Jennifer de Jongh; Reginald W Marsh; Tinte Itinteang; Swee T Tan Journal: Plast Reconstr Surg Glob Open Date: 2020-08-19
Authors: Imogen M Roth; Agadha C Wickremesekera; Susrutha K Wickremesekera; Paul F Davis; Swee T Tan Journal: Front Oncol Date: 2019-08-08 Impact factor: 6.244
Authors: David Ch Tan; Imogen M Roth; Agadha C Wickremesekera; Paul F Davis; Andrew H Kaye; Theo Mantamadiotis; Stanley S Stylli; Swee T Tan Journal: Cells Date: 2019-10-31 Impact factor: 6.600
Authors: Sam Siljee; Bridget Milne; Helen D Brasch; Nicholas Bockett; Josie Patel; Paul F Davis; Andrew Kennedy-Smith; Tinte Itinteang; Swee T Tan Journal: Biomolecules Date: 2021-04-07
Authors: Felix Humphries; Bridget Chang-McDonald; Josie Patel; Nicholas Bockett; Erin Paterson; Paul F Davis; Swee T Tan Journal: Front Oncol Date: 2021-09-21 Impact factor: 6.244