Nathalie Sermondade1, Charlotte Sonigo2, Christophe Sifer3, Sophie Valtat4, Marianne Ziol5, Florence Eustache3, Michaël Grynberg6. 1. Department of Cytogenetic and Reproductive Biology, Hôpital Jean Verdier, Hôpitaux Universitaires Paris-Seine-Saint-Denis, Assistance Publique, Hôpitaux de Paris, Bondy, France. Electronic address: nathalie.sermondade@aphp.fr. 2. Department of Reproductive Medicine and Fertility Preservation, Hôpital Jean Verdier, Hôpitaux Universitaires Paris-Seine-Saint-Denis, Assistance Publique, Hôpitaux de Paris, Bondy, France; Inserm U1185, Université Paris-Sud, Université Paris Saclay, Le Kremlin Bicêtre, France. 3. Department of Cytogenetic and Reproductive Biology, Hôpital Jean Verdier, Hôpitaux Universitaires Paris-Seine-Saint-Denis, Assistance Publique, Hôpitaux de Paris, Bondy, France. 4. Inserm UMR1169, Université Paris-Sud, Université Paris Saclay, Le Kremlin Bicêtre, France. 5. Service d'Anatomie Pathologique, Hôpital Jean Verdier, Hôpitaux Universitaires Paris-Seine-Saint-Denis, Assistance Publique, Hôpitaux de Paris, Bondy, France; Unité de Formation et de Recherche Santé Médecine et Biologie Humaine, Université Paris 13, Communauté d'Universités et Etablissements Sorbonne Paris Cité, Paris, France. 6. Department of Reproductive Medicine and Fertility Preservation, Hôpital Jean Verdier, Hôpitaux Universitaires Paris-Seine-Saint-Denis, Assistance Publique, Hôpitaux de Paris, Bondy, France; Unité de Formation et de Recherche Santé Médecine et Biologie Humaine, Université Paris 13, Communauté d'Universités et Etablissements Sorbonne Paris Cité, Paris, France; Inserm U1133 Université Paris Diderot, Paris, France.
Abstract
OBJECTIVE: To assess whether serum antimüllerian hormone (AMH) levels and antral follicle count (AFC) can predict primordial follicle density within ovarian cortex and the number of oocytes cryopreserved after in vitro maturation (IVM). DESIGN: Retrospective analysis of a case series of patients. SETTING: University hospital. PATIENT(S): Fifty-four women, 18 to 35 years of age, with breast cancer who were candidates for fertility preservation (FP) using ovarian tissue cryopreservation (OTC) associated with oocyte vitrification after unstimulated IVM between July 2013 and December 2016. INTERVENTION(S): Serum AMH levels and transvaginal AFC evaluated before FP, cumulus-oocyte complexes (COC) recovered under ultrasound guidance and incubated for IVM, and ovarian tissue laparoscopically harvested and cryopreserved. MAIN OUTCOME MEASURE(S): Univariate and multivariate analysis between ovarian reserve tests, number of recovered and in vitro matured oocytes, and primordial follicle density histologically obtained within ovarian cortex. RESULT(S): Univariate analysis showed a statistically significant correlation between AMH or AFC and primordial follicle density. Multivariate analysis showed a predominant statistically significant correlation of serum AMH with density. Antimüllerian hormone also correlated with the number of COC and in vitro matured oocytes. CONCLUSION(S): Serum AMH levels may reflect the primordial follicle stockpile and may predict outcomes of IVM and OTC when performed for FP. Further analyses are required to evaluate the relevance of performing such procedures in young women who have low values on ovarian reserve tests.
OBJECTIVE: To assess whether serum antimüllerian hormone (AMH) levels and antral follicle count (AFC) can predict primordial follicle density within ovarian cortex and the number of oocytes cryopreserved after in vitro maturation (IVM). DESIGN: Retrospective analysis of a case series of patients. SETTING: University hospital. PATIENT(S): Fifty-four women, 18 to 35 years of age, with breast cancer who were candidates for fertility preservation (FP) using ovarian tissue cryopreservation (OTC) associated with oocyte vitrification after unstimulated IVM between July 2013 and December 2016. INTERVENTION(S): Serum AMH levels and transvaginal AFC evaluated before FP, cumulus-oocyte complexes (COC) recovered under ultrasound guidance and incubated for IVM, and ovarian tissue laparoscopically harvested and cryopreserved. MAIN OUTCOME MEASURE(S): Univariate and multivariate analysis between ovarian reserve tests, number of recovered and in vitro matured oocytes, and primordial follicle density histologically obtained within ovarian cortex. RESULT(S): Univariate analysis showed a statistically significant correlation between AMH or AFC and primordial follicle density. Multivariate analysis showed a predominant statistically significant correlation of serum AMH with density. Antimüllerian hormone also correlated with the number of COC and in vitro matured oocytes. CONCLUSION(S): Serum AMH levels may reflect the primordial follicle stockpile and may predict outcomes of IVM and OTC when performed for FP. Further analyses are required to evaluate the relevance of performing such procedures in young women who have low values on ovarian reserve tests.
Authors: Ashley A Diaz; Hana Kubo; Nicole Handa; Maria Hanna; Monica M Laronda Journal: Front Endocrinol (Lausanne) Date: 2022-06-10 Impact factor: 6.055
Authors: Ewa Rudnicka; Michał Kunicki; Anna Calik-Ksepka; Katarzyna Suchta; Anna Duszewska; Katarzyna Smolarczyk; Roman Smolarczyk Journal: Int J Mol Sci Date: 2021-11-19 Impact factor: 5.923