Motonari Shibakami1, Kazuhiko Shibata2, Akira Akashi3, Nobuteru Onaka3, Jun Takezaki3, Gen Tsubouchi3, Hiroaki Yoshikawa3. 1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6th, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan. moto.shibakami@aist.go.jp. 2. Artisan Labo, 3-29-7 Nohmidai, Kanazawa, Yokohama, Kanagawa, 236-0056, Japan. 3. Technical Research Center, KOBELCO Eco-Solutions Co., Ltd., 1-1-4 Murotani, Kobe, Hyogo, 651-2241, Japan.
Abstract
PURPOSE: Straight-chain polysaccharides have a greater potential of selectively adsorbing hydrophobic bile salts than resin-based bile salt sequesters because of ionic and hydrophobic interactions; hence, they may possess antidiabetic activity. The feasibility of using cationic polysaccharides made from euglenoid β-1,3-glucan (referred to as paramylon) as potential antidiabetic agents was examined by using in vitro and animal experiments. METHODS: Cationic straight-chain polysaccharides were synthesized from euglenoid polysaccharide and glycidyltrimethylammonium chloride. The effects of administration of the synthetic polysaccharide on metabolic syndrome-related indicators were examined in high-fat diet-induced obesity mice. The degree of adsorption of bile salts by the polysaccharides was evaluated using spectroscopic analysis. RESULTS: Administration of the cationic paramylon derivatives significantly reduced body and mesenteric fat weight in high-fat diet-induced obesity mice. A noteworthy effect was that glucagon-like peptide-1 (GLP-1) secretion was approximately three times higher in diet-induced obesity mice receiving cationic paramylon derivatives than in those receiving cellulose as a control. CONCLUSIONS: Our results indicate that these cationic paramylon derivatives are potential GLP-1 secretagogues suitable for further study.
PURPOSE: Straight-chain polysaccharides have a greater potential of selectively adsorbing hydrophobic bile salts than resin-based bile salt sequesters because of ionic and hydrophobic interactions; hence, they may possess antidiabetic activity. The feasibility of using cationic polysaccharides made from euglenoid β-1,3-glucan (referred to as paramylon) as potential antidiabetic agents was examined by using in vitro and animal experiments. METHODS: Cationic straight-chain polysaccharides were synthesized from euglenoid polysaccharide and glycidyltrimethylammonium chloride. The effects of administration of the synthetic polysaccharide on metabolic syndrome-related indicators were examined in high-fat diet-induced obesitymice. The degree of adsorption of bile salts by the polysaccharides was evaluated using spectroscopic analysis. RESULTS: Administration of the cationic paramylon derivatives significantly reduced body and mesenteric fat weight in high-fat diet-induced obesitymice. A noteworthy effect was that glucagon-like peptide-1 (GLP-1) secretion was approximately three times higher in diet-induced obesitymice receiving cationic paramylon derivatives than in those receiving cellulose as a control. CONCLUSIONS: Our results indicate that these cationic paramylon derivatives are potential GLP-1 secretagogues suitable for further study.
Entities:
Keywords:
GLP-1; antidiabetic agent; bile salt sequestrants; polysaccharide
Authors: E Mannucci; A Ognibene; F Cremasco; G Bardini; A Mencucci; E Pierazzuoli; S Ciani; G Messeri; C M Rotella Journal: Diabetes Care Date: 2001-03 Impact factor: 19.112