Karlygash Abdiyeva1, Nurkeldi Turebekov2, Andrey Dmitrovsky3, Nur Tukhanova4, Anna Shin5, Lyazat Yeraliyeva6, Norbert Heinrich7, Michael Hoelscher8, Ravilya Yegemberdiyeva9, Zhanna Shapiyeva10, Zulfiya Kachiyeva11, Aliya Zhalmagambetova12, Josef Montag13, Gerhard Dobler14, Josua Zinner15, Edith Wagner16, Stefan Frey17, Sandra Essbauer18. 1. Centre for International Health, Ludwig-Maximilian-University, Munich, Germany. Electronic address: karla.abdi@yandex.ru. 2. Centre for International Health, Ludwig-Maximilian-University, Munich, Germany. Electronic address: Nurik_1976@mail.ru. 3. Kazakh National Medical University, Almaty, Kazakhstan. Electronic address: am_dimitr@mail.ru. 4. Centre for International Health, Ludwig-Maximilian-University, Munich, Germany. Electronic address: tukhanovavur@gmail.com. 5. Centre for International Health, Ludwig-Maximilian-University, Munich, Germany; Kazakh National Medical University, Almaty, Kazakhstan. Electronic address: annashin86@gmail.com. 6. Kazakh National Medical University, Almaty, Kazakhstan. Electronic address: elt.niifpm@kaznmu.kz. 7. Division of Infectious Diseases and Tropical Medicine, Ludwig-Maximilians-University, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: heinrich@lrz.uni-muenchen.de. 8. Division of Infectious Diseases and Tropical Medicine, Ludwig-Maximilians-University, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: hoelscher@lrz.uni-muenchen.de. 9. Kazakh National Medical University, Almaty, Kazakhstan. Electronic address: regemberdieva@rambler.ru. 10. Scientific Practical Centre for Sanitary Epidemiological Expertise and Monitoring, Almaty, Kazakhstan. Electronic address: z.shapiyeva@gmail.com. 11. Kazakh National Medical University, Almaty, Kazakhstan. Electronic address: kachieva@gmail.com. 12. German Association for International Cooperation, Almaty, Kazakhstan. Electronic address: aliya.zhalmagambetova@giz.de. 13. Faculty of Law, Charles University, Prague, Czech Republic. Electronic address: josef.Montag@gmail.com. 14. Bundeswehr Institute of Microbiology, Department Virology and Intracellular Agents, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: gerharddobler@bundeswehr.org. 15. Bundeswehr Institute of Microbiology, Department Virology and Intracellular Agents, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: josuazinner@bundeswehr.org. 16. Bundeswehr Institute of Microbiology, Department Virology and Intracellular Agents, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: edithwagner@bundeswehr.org. 17. Bundeswehr Institute of Microbiology, Department Virology and Intracellular Agents, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: stefan1frey@bundeswehr.org. 18. Bundeswehr Institute of Microbiology, Department Virology and Intracellular Agents, German Centre for Infection Research, Munich Partner Site, Munich, Germany. Electronic address: sandraessbauer@bundeswehr.org.
Abstract
OBJECTIVES: The aim of this study was to detect the seroprevalence of Crimean-Congo haemorrhagic fever virus (CCHFV) in patients with fever of unknown origin (FUO) in endemic (Kyzylorda) and non-endemic (Almaty) oblasts of Kazakhstan. METHODS: Paired serum samples from 802 patients with FUO were collected. Serum samples were investigated by ELISA to detect IgG and IgM antibodies against CCHFV. Sera with suspected acute infection were further investigated by RT-PCR to detect the viral RNA. RESULTS: IgG antibodies were detected in 12.7% of the sera from both oblasts. Acute infection was shown by IgM ELISA in four patients from Kyzylorda, with only one developing severe CCHF. Viral RNA was found by RT-PCR in the other three patients' sera. Phylogenetic analysis of partial L and S segments revealed CCHFV genotype Asia 2 and a possible reassortment between the genotypes Asia 1/Asia 2. Animal husbandry, such as working with cattle and horses, was significantly associated with CCHFV seropositivity. CONCLUSIONS: The antibodies and viral RNA detected in sera indicate that mild or even asymptomatic CCHFV infections are presented in Kazakhstan. This study describes the circulation of CCHFV in the so far non-endemic Almaty oblast for the first time. In conclusion, physicians treating patients with FUO in Kazakhstan should be aware of mild CCHF.
OBJECTIVES: The aim of this study was to detect the seroprevalence of Crimean-Congo haemorrhagic fever virus (CCHFV) in patients with fever of unknown origin (FUO) in endemic (Kyzylorda) and non-endemic (Almaty) oblasts of Kazakhstan. METHODS: Paired serum samples from 802 patients with FUO were collected. Serum samples were investigated by ELISA to detect IgG and IgM antibodies against CCHFV. Sera with suspected acute infection were further investigated by RT-PCR to detect the viral RNA. RESULTS: IgG antibodies were detected in 12.7% of the sera from both oblasts. Acute infection was shown by IgM ELISA in four patients from Kyzylorda, with only one developing severe CCHF. Viral RNA was found by RT-PCR in the other three patients' sera. Phylogenetic analysis of partial L and S segments revealed CCHFV genotype Asia 2 and a possible reassortment between the genotypes Asia 1/Asia 2. Animal husbandry, such as working with cattle and horses, was significantly associated with CCHFV seropositivity. CONCLUSIONS: The antibodies and viral RNA detected in sera indicate that mild or even asymptomatic CCHFV infections are presented in Kazakhstan. This study describes the circulation of CCHFV in the so far non-endemic Almaty oblast for the first time. In conclusion, physicians treating patients with FUO in Kazakhstan should be aware of mild CCHF.
Authors: Jonathan Bryant-Genevier; Yekaterina Bumburidi; Lilit Kazazian; Victoria Seffren; Jennifer R Head; Dmitriy Berezovskiy; Bakhytkul Zhakipbayeva; Stephanie J Salyer; Barbara Knust; John D Klena; Cheng-Feng Chiang; Gulfaira Mirzabekova; Kumisbek Rakhimov; Jandar Koekeev; Kanatbek Kartabayev; Seydigapbar Mamadaliyev; Marta Guerra; Curtis Blanton; Trevor Shoemaker; Daniel Singer; Daphne B Moffett Journal: Am J Trop Med Hyg Date: 2022-04-04 Impact factor: 3.707
Authors: Kulyaisan T Sultankulova; Gaukhar O Shynybekova; Nurlan S Kozhabergenov; Nazym N Mukhami; Olga V Chervyakova; Yerbol D Burashev; Kunsulu D Zakarya; Aziz K Nakhanov; Kainar B Barakbayev; Mukhit B Orynbayev Journal: Pathogens Date: 2022-07-27