Yabin Hu1,2, Qing Yang3, Yanyan Gao4, Xuexin Guo5, Yongjian Liu6, Can Li7, Yanmeng Du8, Lei Gao9, Dezheng Sun10, Congcong Zhu1, Mi Yan1. 1. Department of Radiology, Affiliated Hospital (Laoshan Hospital) of Qingdao University, Qingdao, 266000, Shandong, China. 2. Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Medical Imaging, Shanghai, China. 3. Department of Radiology, Affiliated Hospital (Laoshan Hospital) of Qingdao University, Qingdao, 266000, Shandong, China. yangqingqdfy@126.com. 4. Department of Endocrinology, Affiliated Hospital (Laoshan Hospital) of Qingdao University, Qingdao, Shandong, China. 5. Department of Radiology, Dongying People's Hospital, Dongying, Shandong, China. 6. Department of Radiology, Hiser Medical Center of Qingdao, Qingdao, Shandong, China. 7. Department of CT, Juancheng People's Hospital, Juancheng, Heze, Shandong, China. 8. CT scan Room, Jinan Fourth Hospital, Jinan, Shandong, China. 9. Department of CT, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei, China. 10. Department of Radiology, Qingdao Municipal Hospital, Qingdao, Shandong, China.
Abstract
OBJECTIVE: To assess hemodynamic changes related to acute gouty knee arthritis in a rabbit with CT perfusion (CTP) METHODS: Forty-two rabbits were randomly separated into two groups: the treated group of 30 and the control group of 12. The right knee was injected with monosodium urate solution and polymyxin in the treated group and saline and polymyxin in the control group. At 2, 16, 32, 48, 60, and 72 h after injection, five rabbits from the treated group and two rabbits from the control group were selected for CTP. At each time point, blood flow (BF), blood volume (BV), and clearance rate (CL) were measured, and microvessel density (MVD) was evaluated with a microscope. RESULTS: In the treated group, BF, BV, CL, and MVD were significantly higher than in the control group (p < 0.001). Differences within paired comparison of BV, BF, CL, and MVD were all significant (all p < 0.001). Peak time of BV, BF, and MVD was 32 h and 48 h for CL. After multivariate stepwise linear regression analysis, BV was linearly associated with MVD and vice versa, which also applied to BF with MVD and BF with CL, separately. The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL. CONCLUSION: CTP in this rabbit knee model accurately detected hemodynamic changes during a gouty attack. KEY POINTS: • Acute gouty arthritis can be evaluated with CTP in a rabbit knee model. • Following injection of MSU crystals, producing an acute gouty attack, CTP successfully assessed hemodynamic changes. • The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL.
OBJECTIVE: To assess hemodynamic changes related to acute gouty knee arthritis in a rabbit with CT perfusion (CTP) METHODS: Forty-two rabbits were randomly separated into two groups: the treated group of 30 and the control group of 12. The right knee was injected with monosodium urate solution and polymyxin in the treated group and saline and polymyxin in the control group. At 2, 16, 32, 48, 60, and 72 h after injection, five rabbits from the treated group and two rabbits from the control group were selected for CTP. At each time point, blood flow (BF), blood volume (BV), and clearance rate (CL) were measured, and microvessel density (MVD) was evaluated with a microscope. RESULTS: In the treated group, BF, BV, CL, and MVD were significantly higher than in the control group (p < 0.001). Differences within paired comparison of BV, BF, CL, and MVD were all significant (all p < 0.001). Peak time of BV, BF, and MVD was 32 h and 48 h for CL. After multivariate stepwise linear regression analysis, BV was linearly associated with MVD and vice versa, which also applied to BF with MVD and BF with CL, separately. The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL. CONCLUSION:CTP in this rabbit knee model accurately detected hemodynamic changes during a gouty attack. KEY POINTS: • Acute gouty arthritis can be evaluated with CTP in a rabbit knee model. • Following injection of MSU crystals, producing an acute gouty attack, CTP successfully assessed hemodynamic changes. • The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL.
Authors: R Clive Landis; Darshna R Yagnik; Oliver Florey; Pandelis Philippidis; Vivien Emons; Justin C Mason; Dorian O Haskard Journal: Arthritis Rheum Date: 2002-11
Authors: Martijn R Meijerink; Jan Hein T M van Waesberghe; Cors van Schaik; Epie Boven; Astrid A M van der Veldt; Petrousjka van den Tol; Sybren Meijer; Cornelis van Kuijk Journal: Ultrasound Med Biol Date: 2010-10 Impact factor: 2.998