Tahnee J Dening1,2, Paul Joyce1,3, Miia Kovalainen1,4, Hanna Gustafsson1,2, Clive A Prestidge5,6. 1. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, 5001, Australia. 2. ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of South Australia, Adelaide, South Australia, 5001, Australia. 3. Department of Physics, Chalmers University of Technology, SE-412 96, Gothenburg, Sweden. 4. Research Unit of Biomedicine and Biocenter of Oulu, Faculty of Medicine, University of Oulu, P.O. Box 5000, FI-90014, Oulo, Finland. 5. School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, South Australia, 5001, Australia. clive.prestidge@unisa.edu.au. 6. ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, University of South Australia, Adelaide, South Australia, 5001, Australia. clive.prestidge@unisa.edu.au.
Abstract
PURPOSE: To explore the feasibility of spray dried smectite clay particles fabricated from montmorillonite or laponite materials for adsorbing dietary lipids and reducing rodent weight gain in vivo. METHODS: Spray dried montmorillonite (SD-MMT) and spray dried laponite (SD-LAP) particles were prepared via spray drying. Particle morphology, surface area and redispersion/aggregation properties in aqueous media were characterized. The ability of SD-MMT and SD-LAP particles to inhibit lipid digestion kinetics and adsorb lipid species from solution was assessed during in vitro lipolysis using proton nuclear magnetic resonance analysis. SD-MMT and SD-LAP particles were dosed to rodents fed a high-fat diet and their effect on body weight gain was evaluated. RESULTS: Both SD-MMT and SD-LAP particles adsorbed significant quantities of medium chain triglycerides and lipolytic products from solution during in vitro lipolysis. At a concentration of 50% w/w relative to lipid content, SD-MMT and SD-LAP particles adsorbed 42% and 94% of all lipid species, respectively. SD-MMT and SD-LAP particles also reduced the extent of rodent weight gain relative to the negative control treatment group and performed similarly to orlistat via an alternate mechanism of action. CONCLUSIONS: Spray dried smectite clay particles (SD-MMT and SD-LAP) with significant adsorptive capacities for dietary lipids and digestion products were successfully fabricated. These particles may be developed as novel anti-obesity treatments with fewer adverse effects than currently marketed treatment options.
PURPOSE: To explore the feasibility of spray dried smectite clay particles fabricated from montmorillonite or laponite materials for adsorbing dietary lipids and reducing rodent weight gain in vivo. METHODS: Spray dried montmorillonite (SD-MMT) and spray dried laponite (SD-LAP) particles were prepared via spray drying. Particle morphology, surface area and redispersion/aggregation properties in aqueous media were characterized. The ability of SD-MMT and SD-LAP particles to inhibit lipid digestion kinetics and adsorb lipid species from solution was assessed during in vitro lipolysis using proton nuclear magnetic resonance analysis. SD-MMT and SD-LAP particles were dosed to rodents fed a high-fat diet and their effect on body weight gain was evaluated. RESULTS: Both SD-MMT and SD-LAP particles adsorbed significant quantities of medium chain triglycerides and lipolytic products from solution during in vitro lipolysis. At a concentration of 50% w/w relative to lipid content, SD-MMT and SD-LAP particles adsorbed 42% and 94% of all lipid species, respectively. SD-MMT and SD-LAP particles also reduced the extent of rodent weight gain relative to the negative control treatment group and performed similarly to orlistat via an alternate mechanism of action. CONCLUSIONS: Spray dried smectite clay particles (SD-MMT and SD-LAP) with significant adsorptive capacities for dietary lipids and digestion products were successfully fabricated. These particles may be developed as novel anti-obesity treatments with fewer adverse effects than currently marketed treatment options.
Authors: J O Hill; J Hauptman; J W Anderson; K Fujioka; P M O'Neil; D K Smith; J H Zavoral; L J Aronne Journal: Am J Clin Nutr Date: 1999-06 Impact factor: 7.045
Authors: Tahnee J Dening; Paul Joyce; Jessie L Webber; David A Beattie; Clive A Prestidge Journal: J Colloid Interface Sci Date: 2018-08-07 Impact factor: 8.128