Literature DB >> 3051781

Detection of ABO blood group-active glycolipids extracted from red cell membrane and heat hematoma using TLC-immunostaining.

M Ota1, H Fukushima, I Yonemura, H Hasekura.   

Abstract

Glycolipids extracted from groups A, B, and O erythrocytes were developed on thin-layer plates; their ABO blood group antigenicities were detected by immunostaining method using avidin-biotin-complex (ABC). Among series of glycolipids of different flow rates, antigen-specific staining was observed in five bands from group A1 erythrocytes, four bands from group B, and two bands from group O. Monoclonal anti-A, -B, and -H antibodies specifically stained glycolipids from A1, B, and O erythrocytes, respectively. ABO blood grouping was possible from 5 g of epidural heat hematoma of a charred body by this method. ABC immunostaining on thin-layer chromatography is a useful and reliable method for ABO blood grouping in forensic practice.

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Year:  1988        PMID： 3051781     DOI： 10.1007/BF00200762

Source DB:  PubMed          Journal:  Z Rechtsmed        ISSN： 0044-3433


  16 in total

1.  Blood group determination of severely charred bodies--the effects of heating on the blood group activity of A, B, AB and O(H) active glycolipids and A and B active glycoproteins.

Authors:  T Nagano; T Tsuji; K Ieda
Journal:  J Forensic Sci Soc       Date:  1976-04

2.  Repetitive A epitope (type 3 chain A) defined by blood group A1-specific monoclonal antibody TH-1: chemical basis of qualitative A1 and A2 distinction.

Authors:  H Clausen; S B Levery; E Nudelman; S Tsuchiya; S Hakomori
Journal:  Proc Natl Acad Sci U S A       Date:  1985-02       Impact factor: 11.205

Review 3.  Blood group ABH and Ii antigens of human erythrocytes: chemistry, polymorphism, and their developmental change.

Authors:  S Hakomori
Journal:  Semin Hematol       Date:  1981-01       Impact factor: 3.851

4.  Detection of glycolipid ligands by direct binding of carbohydrate-binding proteins to thin-layer chromatograms.

Authors:  J L Magnani; M Brockhaus; D F Smith; V Ginsburg
Journal:  Methods Enzymol       Date:  1982       Impact factor: 1.600

5.  The use of avidin-biotin interaction in immunoenzymatic techniques.

Authors:  J L Guesdon; T Ternynck; S Avrameas
Journal:  J Histochem Cytochem       Date:  1979-08       Impact factor: 2.479

6.  Characterization of B and H blood-group active glycosphingolopids from human B erythrocyte membranes.

Authors:  P Hanfland
Journal:  Chem Phys Lipids       Date:  1975-11       Impact factor: 3.329

7.  On neutral fucoglycolipids having long, branched carbohydrate chains: H-active and I-active glycosphingolipids of human erythrocyte membranes.

Authors:  K Watanabe; R A Laine; S I Hakomori
Journal:  Biochemistry       Date:  1975-06-17       Impact factor: 3.162

8.  Structures of branched blood group A-active glycosphingolipids in human erythrocytes and polymorphism of A- and H-glycolipids in A1 and A2 subgroups.

Authors:  M N Fukuda; S Hakomori
Journal:  J Biol Chem       Date:  1982-01-10       Impact factor: 5.157

9.  Purification and structures of branched blood-group-B-active glycosphingolipids from human erythrocyte membranes.

Authors:  P Hanfland; M Kordowicz; H Niermann; H Egge; U Dabrowski; J Peter-Katalinic; J Dabrowski
Journal:  Eur J Biochem       Date:  1984-12-17

10.  Blood group A glycolipid (Ax) with globo-series structure which is specific for blood group A1 erythrocytes: one of the chemical bases for A1 and A2 distinction.

Authors:  H Clausen; K Watanabe; R Kannagi; S B Levery; E Nudelman; Y Arao-Tomono; S Hakomori
Journal:  Biochem Biophys Res Commun       Date:  1984-10-30       Impact factor: 3.575
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