| Literature DB >> 30517709 |
Wataru Kobayashi1,2, Enwei Liu2, Hajime Ishii2, Sachihiro Matsunaga3, Peter Schlögelhofer4, Hitoshi Kurumizaka1,2.
Abstract
In eukaryotes, homologous recombination plays a pivotal role in both genome maintenance and generation of genetic diversity. Eukaryotic RecA homologues, RAD51 and DMC1, are key proteins in homologous recombination that promote pairing between homologous DNA sequences. Arabidopsis thaliana is a prominent model plant for studying eukaryotic homologous recombination. However, A. thaliana RAD51 and DMC1 have not been biochemically characterized. In the present study, we purified A. thaliana RAD51 (AtRAD51) and DMC1 (AtDMC1). Biochemical analyses revealed that both AtRAD51 and AtDMC1 possess ATP hydrolyzing activity, filament formation activity and homologous pairing activity in vitro. We then compared the homologous pairing activities of AtRAD51 and AtDMC1 with those of the Oryza sativa and Homo sapiens RAD51 and DMC1 proteins.Entities:
Keywords: zzm321990 Arabidopsis thalianazzm321990 ; DMC1; RAD51; homologous pairing; homologous recombination
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Year: 2019 PMID： 30517709 DOI： 10.1093/jb/mvy105
Source DB: PubMed Journal: J Biochem ISSN： 0021-924X Impact factor: 3.387