Betânia Souza Monteiro1, Bianka Souza Dos Santos2, Bruna Lopes de Almeida2, Emy Hiura2, Wagner Alexey Back Fiorio3, Gisele Pereira Valdetaro2, Dariele Vieira Gonçalves2, Caroline Saraiva Silva4, Tatiana Champion5, Daniela Campagnol6. 1. Full Professor, Stem Cell and Cellular Therapy Laboratory, Animal Science Program, Universidade Vila Velha (UVV), Brazil. Conception and design of the study, manuscript writing, critical revision, final approval. 2. Veterinary Medical, Stem Cell and Cellular Therapy Laboratory, Animal Science Program, UVV, Vila Velha-ES, Brazil. Technical procedures, acquisition of data, manuscript preparation and writing. 3. Veterinay Medical, Advanced Imaging Center and Veterinary Pathology, Vila Velha-ES, Brazil. Histopathological examinations. 4. Veterinay Medical, PetScan Diagnoses Center, Vila Velha-ES, Brazil. Imagens examinations. 5. Assistant Professor, Veterinary Department, Universidade Federal da Fronteira Sul (UFFS), Realeza-PR, Brazil. Statistics analysis, critical revision, final approval. 6. Veterinay Medical, Animal Experimentation Division, Clinical Hospital, Universidade Federal do Rio Grande do Sul (UFRS), Porto Alegre-RS, Brazil. Substantive scientific and intellectual contributions to the study, critical revision.
Abstract
PURPOSE: To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell. METHODS: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies. RESULTS: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury. CONCLUSION: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.
PURPOSE: To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell. METHODS: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal). After the treatments were performed, at 8 and 31 days, four animals in each group were subjected to left nephrectomy for histological studies. RESULTS: Histologically, a higher amount of cell debris and tubules devoid of the epithelium and a higher degree of necrosis were observed in the groups treated with PBS, as opposed to a low degree of necrosis and higher tubular vascularization in the groups treated with ADSC, particularly in the group treated with intrarenal ADSC 48 hours after injury. CONCLUSION: The transplantation of ADSC positively contributed to the replacement of necrotic tissue by renal tubular cells, vascularization of the renal parenchyma, and restoration of the organ function.