Brian Dean1,2, Andrew Tsatsanis3,4, Linh Q Lam3,5, Elizabeth Scarr1,6, James A Duce3,4,7. 1. The Molecular Psychiatry Laboratory, The Florey Institute of Neuroscience and Mental Health, Parkville, Australia. 2. The Centre for Mental Health, the Faculty of Health, Arts and Design, Swinburne University, Hawthorne, Australia. 3. Melbourne Dementia Research Centre, The Florey Institute of Neuroscience and Mental Health, Parkville, Australia. 4. School of Biomedical Sciences, University of Leeds, Leeds, UK. 5. Neuropharmacology Laboratory, Department of Pharmacology and Therapeutics, The University of Melbourne, Parkville, Australia. 6. Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Australia. 7. The ALBORADO Drug Discovery Institute, University of Cambridge, Cambridge, UK.
Abstract
Objectives: The objective of this study was to determine whether a breakdown in proteins regulating cortical iron homeostasis could be involved in the pathophysiology of mood disorders. Methods: Levels of select proteins responsible for cortical iron transport were quantitated by Western blotting of Brodmann's (BA) areas 6 and 10 from patients with major depressive disorder (n = 13), bipolar disorder (n = 12) and age/sex matched controls (n = 13). Results: We found the inactive form of ceruloplasmin was lower in BA 6 from males compared to females. Levels of copper containing ceruloplasmin was lower in BA 6 from suicide completers whilst levels of amyloid precursor protein, TAU and transferrin were higher in BA 10 from those individuals. The level of prion protein was lower in BA 6 from subjects with major depressive disorder.Conclusions: Our data suggests that perturbation in cortical iron transport proteins is not prevalent in mood disorders. By contrast, our data suggests changes in iron transport proteins in BA 6 and BA 10 are present after suicide completion. If these changes were present before death, they could have had a role in the genesis of the contemplation and completion of suicide.
Objectives: The objective of this study was to determine whether a breakdown in proteins regulating cortical iron homeostasis could be involved in the pathophysiology of mood disorders. Methods: Levels of select proteins responsible for cortical iron transport were quantitated by Western blotting of Brodmann's (BA) areas 6 and 10 from patients with major depressive disorder (n = 13), bipolar disorder (n = 12) and age/sex matched controls (n = 13). Results: We found the inactive form of ceruloplasmin was lower in BA 6 from males compared to females. Levels of copper containing ceruloplasmin was lower in BA 6 from suicide completers whilst levels of amyloid precursor protein, TAU and transferrin were higher in BA 10 from those individuals. The level of prion protein was lower in BA 6 from subjects with major depressive disorder.Conclusions: Our data suggests that perturbation in cortical iron transport proteins is not prevalent in mood disorders. By contrast, our data suggests changes in iron transport proteins in BA 6 and BA 10 are present after suicide completion. If these changes were present before death, they could have had a role in the genesis of the contemplation and completion of suicide.
Entities:
Keywords:
Iron regulatory proteins; bipolar disorder; cortex; major depressive disorder; prion protein
Authors: Jenessa N Johnston; Darcy Campbell; Hector J Caruncho; Ioline D Henter; Elizabeth D Ballard; Carlos A Zarate Journal: Int J Neuropsychopharmacol Date: 2022-03-17 Impact factor: 5.176
Authors: Ana Martín-Sánchez; Janet Piñero; Lara Nonell; Magdalena Arnal; Elena M Ribe; Alejo Nevado-Holgado; Simon Lovestone; Ferran Sanz; Laura I Furlong; Olga Valverde Journal: Alzheimers Res Ther Date: 2021-04-02 Impact factor: 6.982