| Literature DB >> 30510069 |
Noah Sorrelle1, Debolina Ganguly1, Adrian T A Dominguez1, Yuqing Zhang1, Huocong Huang1, Lekh N Dahal2, Natalie Burton1, Arturas Ziemys3, Rolf A Brekken4,5.
Abstract
Immune profiling of tissue through multiplex immunohistochemistry is important for the investigation of immune cell dynamics, and it can contribute to disease prognosis and evaluation of treatment response in cancer patients. However, protocols for mouse formalin-fixed, paraffin-embedded tissue have been less successful. Given that formalin fixation and paraffin embedding remains the most common preparation method for processing mouse tissue, this has limited the options to study the immune system and the impact of novel therapeutics in preclinical models. In an attempt to address this, we developed an improved immunohistochemistry protocol with a more effective Ag-retrieval buffer. We also validated 22 Abs specific for mouse immune cell markers to distinguish B cells, T cells, NK cells, macrophages, dendritic cells, and neutrophils. In addition, we designed and tested novel strategies to identify immune cells for which unique Abs are currently not available. Last, in the 4T1 model of breast cancer, we demonstrate the utility of our protocol and Ab panels in the quantitation and spatial distribution of immune cells.Entities:
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Year: 2018 PMID： 30510069 PMCID： PMC6310091 DOI： 10.4049/jimmunol.1800878
Source DB: PubMed Journal: J Immunol ISSN： 0022-1767 Impact factor: 5.422