Xiaohan Wang1,2, Ling Liu3, Shanmao Mou4, Huishan Zhao5, Jianye Fang5, Yanjie Xiang6, Tong Zhao2, Tongye Sha2, Jie Ding2, Cuifang Hao5. 1. Shandong University, Jinan, Shandong, China. 2. Department of Obstetrics, Rizhao Hospital of Traditional Chinese Medicine, Rizhao, Shandong, China. 3. Department of Reproductive Medicine, The Affiliated Weihai Hospital of Qingdao University, Weihai, Shandong, China. 4. Department of Encephalopathy, Rizhao Hospital of Traditional Chinese Medicine, Rizhao, Shandong, China. 5. Department of Reproductive Medicine, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China. 6. Department of Reproductive Medicine, People's Hospital of Rizhao, Rizhao, Shandong, China.
Abstract
BACKGROUND: Platelet-rich plasma (PRP) contains abundant growth factors and is gradually used in the field of reproduction. A thin endometrium is recognized as a critical factor in embryo implantation failure. Endometrial mesenchymal stem cells (EnMSCs), which were isolated from human menstrual blood, are highly proliferative and show multiple differentiation capacity. The current study was to investigate the effect of PRP on the proliferation and migration of EnMSCs, and the effectiveness of PRP in the treatment of patients with thin endometrium. MATERIALS AND METHODS: EnMSCs were treated with PRP in vitro, followed by measuring cell proliferation, migration, and adhesion by using CCK8, scratch, and adhesion test, respectively. Twenty patients undergoing in vitro fertilization (IVF) with refractory thin endometrium history were given PRP by infusion into the uterine cavity after the treatment of hormone replacement therapy (HRT). RESULTS: All components of PRP significantly stimulated the growth, migration, and adhesion of EnMSCs when compared with the negative control. Cell proliferation and migration were induced by PRP in a dose-dependent manner with maximum proliferation at a 2% PRP dose. The clinical data showed that successful endometrial expansion and pregnancy were discovered in 12 patients after PRP infusion, and the pregnancy rate increased to 60%. CONCLUSION: Intrauterine PRP infusion represents a new way for female patients with thin endometrium with poor response. This study lays the foundations for the potential treatment of thin endometrium with PRP in vivo.
BACKGROUND: Platelet-rich plasma (PRP) contains abundant growth factors and is gradually used in the field of reproduction. A thin endometrium is recognized as a critical factor in embryo implantation failure. Endometrial mesenchymal stem cells (EnMSCs), which were isolated from human menstrual blood, are highly proliferative and show multiple differentiation capacity. The current study was to investigate the effect of PRP on the proliferation and migration of EnMSCs, and the effectiveness of PRP in the treatment of patients with thin endometrium. MATERIALS AND METHODS: EnMSCs were treated with PRP in vitro, followed by measuring cell proliferation, migration, and adhesion by using CCK8, scratch, and adhesion test, respectively. Twenty patients undergoing in vitro fertilization (IVF) with refractory thin endometrium history were given PRP by infusion into the uterine cavity after the treatment of hormone replacement therapy (HRT). RESULTS: All components of PRP significantly stimulated the growth, migration, and adhesion of EnMSCs when compared with the negative control. Cell proliferation and migration were induced by PRP in a dose-dependent manner with maximum proliferation at a 2% PRP dose. The clinical data showed that successful endometrial expansion and pregnancy were discovered in 12 patients after PRP infusion, and the pregnancy rate increased to 60%. CONCLUSION: Intrauterine PRP infusion represents a new way for female patients with thin endometrium with poor response. This study lays the foundations for the potential treatment of thin endometrium with PRP in vivo.
Authors: Min Kyoung Kim; Jung Ah Yoon; Sook Young Yoon; Mira Park; Woo Sik Lee; Sang Woo Lyu; Haengseok Song Journal: Cells Date: 2022-05-05 Impact factor: 7.666