| Literature DB >> 3050494 |
Abstract
The immunofluorescent staining of kinetochores in micronuclei with antikinetochore antibodies was used to develop an in vitro assay for aneuploidy-inducing agents. The results show that about 80% of micronuclei induced by either colchicine or chloral hydrate contained kinetochores; only 9% of X-ray-induced micronuclei reacted positively to the antibody. These findings indicate that the in vitro micronucleus assay coupled with immunofluorescent staining of kinetochores can be a useful method for assessing the ability of chemicals to induce aneuploidy and/or chromosome aberrations.Entities:
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Year: 1988 PMID： 3050494 DOI： 10.1016/0165-1161(88)90030-1
Source DB: PubMed Journal: Mutat Res ISSN： 0027-5107 Impact factor: 2.433