Sonia E Trojan1, Monika Piwowar2, Barbara Ostrowska1, Piotr Laidler1, Kinga A Kocemba-Pilarczyk3. 1. Chair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University - Medical College, Cracow, Poland. 2. Department of Bioinformatics and Telemedicine, Faculty of Medicine, Jagiellonian University - Medical College, Cracow, Poland. 3. Chair of Medical Biochemistry, Faculty of Medicine, Jagiellonian University - Medical College, Cracow, Poland kinga.kocemba@uj.edu.pl.
Abstract
BACKGROUND/AIM: Most melanomas develop in hypoxic conditions. Since hypoxia via HIF-1 induces glycolysis, a process essential for malignant melanoma growth/survival, the goal of this study was to analyze the influence of hypoxia on the expression of HIF-1 target genes involved in glucose metabolism. MATERIALS AND METHODS: The response of melanoma cell lines to hypoxic conditions was analyzed by RT-PCR and western blotting. A Kaplan-Meier survival analysis for patients with high and low expression level of PFKFB4 was performed. Further analysis of patients' data was performed using the R/Bioconductor environment. RESULTS: Induction of PFKFB4 gene expression can be considered a crucial mechanism behind glycolysis enhancement in hypoxic melanoma cells. Analysis of a publicly available database revealed that high PFKFB4 expression contributes to poor prognosis of melanoma patients. CONCLUSION: Currently available anti-melanoma therapeutic strategies may significantly benefit from agents targeting PFKFB4 activity. Copyright
BACKGROUND/AIM: Most melanomas develop in hypoxic conditions. Since hypoxia via HIF-1 induces glycolysis, a process essential for malignant melanoma growth/survival, the goal of this study was to analyze the influence of hypoxia on the expression of HIF-1 target genes involved in glucose metabolism. MATERIALS AND METHODS: The response of melanoma cell lines to hypoxic conditions was analyzed by RT-PCR and western blotting. A Kaplan-Meier survival analysis for patients with high and low expression level of PFKFB4 was performed. Further analysis of patients' data was performed using the R/Bioconductor environment. RESULTS: Induction of PFKFB4 gene expression can be considered a crucial mechanism behind glycolysis enhancement in hypoxic melanoma cells. Analysis of a publicly available database revealed that high PFKFB4 expression contributes to poor prognosis of melanomapatients. CONCLUSION: Currently available anti-melanoma therapeutic strategies may significantly benefit from agents targeting PFKFB4 activity. Copyright
Authors: Sonia E Trojan; Michał J Markiewicz; Katarzyna Leśkiewicz; Kinga A Kocemba-Pilarczyk Journal: Cancer Cell Int Date: 2019-11-14 Impact factor: 5.722