Hiroaki Wakiyama1,2,3, Takaaki Masuda1, Yushi Motomura1,2,3, Qingjiang Hu1, Taro Tobo4, Hidetoshi Eguchi1, Katsumi Sakamoto2, Masakazu Hirakawa2, Hiroshi Honda3, Koshi Mimori5. 1. Department of Surgery, Kyushu University Beppu Hospital, Oita, Japan. 2. Department of Radiology, Kyushu University Beppu Hospital, Oita, Japan. 3. Department of Clinical Radiology, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. 4. Clinical Laboratory Medicine and Pathology, Kyushu University Beppu Hospital, Oita, Japan. 5. Department of Surgery, Kyushu University Beppu Hospital, Oita, Japan kmimori@beppu.kyushu-u.ac.jp.
Abstract
BACKGROUND/AIM: The cytolytic activity (CYT) score is a new index of cancer immunity calculated from the mRNA expression levels of GZMA and PRF1. We assessed the clinical significance of the CYT score in HCC. MATERIALS AND METHODS: The calculated CYT scores of peripheral blood cells (GSE24759), cell lines (CCLE) and HCC tissues (TCGA, GSE14520 and Kyushu cohorts) were assessed. Then, immunohistochemical analysis (IHC) of GZMA and PRF1 was performed. RESULTS: The CYT scores of HCC tissues were lower than those of non-cancerous tissues. The 5-year recurrence-free survival of patients with low CYT scores was significantly shorter than that of patients with high CYT scores. Multivariate analysis indicated that the CYT score was an independent prognostic factor for RFS in TCGA and GSE14520 cohorts. CONCLUSION: CYT score could be a useful prognostic biomarker in HCC, possibly through reflecting the host immune status. Copyright
BACKGROUND/AIM: The cytolytic activity (CYT) score is a new index of cancer immunity calculated from the mRNA expression levels of GZMA and PRF1. We assessed the clinical significance of the CYT score in HCC. MATERIALS AND METHODS: The calculated CYT scores of peripheral blood cells (GSE24759), cell lines (CCLE) and HCC tissues (TCGA, GSE14520 and Kyushu cohorts) were assessed. Then, immunohistochemical analysis (IHC) of GZMA and PRF1 was performed. RESULTS: The CYT scores of HCC tissues were lower than those of non-cancerous tissues. The 5-year recurrence-free survival of patients with low CYT scores was significantly shorter than that of patients with high CYT scores. Multivariate analysis indicated that the CYT score was an independent prognostic factor for RFS in TCGA and GSE14520 cohorts. CONCLUSION: CYT score could be a useful prognostic biomarker in HCC, possibly through reflecting the host immune status. Copyright