Nagore Ambrosio1, María José Marín2, Estefanía Laguna3, David Herrera4, Mariano Sanz5, Elena Figuero6. 1. Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain. Electronic address: nambrosio@ucm.es. 2. Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain. Electronic address: mjmarin@ucm.es. 3. Section of Post-Graduate Periodontology - Faculty of Odontology, University Complutense, Madrid, Spain. Electronic address: estefanialaguna@periocentrum.com. 4. Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain. Electronic address: davidher@ucm.es. 5. Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain. Electronic address: marsan@ucm.es. 6. Etiology and Therapy of Periodontal Diseases (ETEP) Research Group, University Complutense, Madrid, Spain. Electronic address: elfiguer@ucm.es.
Abstract
OBJECTIVE: This study was aimed to compare the presence and amounts of bacteremia induced by interdental brushing in periodontally healthy (H) and periodontitis (P) individuals using culture based (direct culture [DC]) and molecular based techniques (real-time quantitative polymerase chain reaction [qPCR]) in a cross-sectional study model. MATERIALS AND METHODS: After a full mouth periodontal evaluation, blood samples were taken before and 1 min after professionally-administered interdental brushing. These samples were analyzed by DC and qPCR, targeting Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Subgingival samples were also collected and analyzed. Student t-test, chi-squar tests and correlations were used for analyzing the data. RESULTS: Thirty individuals per group were included. P. gingivalis and A. actinomycetemcomitans were detected with qPCR methods, but not with DC. At baseline, bacteremia was observed in 5 P patients (16.7%) and in 2 H individuals (6.6%) (p = 0.421). After interdental brushing, bacteremia was only observed in 2 P patients (6.6%) (p = 0.901). A positive correlation between subgingival and blood levels of A. actinomycetemcomitans was observed (r = 0.3; p = 0.013). CONCLUSION: Bacteremia related to A. actinomycetemcomitans and P. gingivalis did not significantly increase after a single session of use of interdental brushes.
OBJECTIVE: This study was aimed to compare the presence and amounts of bacteremia induced by interdental brushing in periodontally healthy (H) and periodontitis (P) individuals using culture based (direct culture [DC]) and molecular based techniques (real-time quantitative polymerase chain reaction [qPCR]) in a cross-sectional study model. MATERIALS AND METHODS: After a full mouth periodontal evaluation, blood samples were taken before and 1 min after professionally-administered interdental brushing. These samples were analyzed by DC and qPCR, targeting Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans. Subgingival samples were also collected and analyzed. Student t-test, chi-squar tests and correlations were used for analyzing the data. RESULTS: Thirty individuals per group were included. P. gingivalis and A. actinomycetemcomitans were detected with qPCR methods, but not with DC. At baseline, bacteremia was observed in 5 P patients (16.7%) and in 2 H individuals (6.6%) (p = 0.421). After interdental brushing, bacteremia was only observed in 2 P patients (6.6%) (p = 0.901). A positive correlation between subgingival and blood levels of A. actinomycetemcomitans was observed (r = 0.3; p = 0.013). CONCLUSION:Bacteremia related to A. actinomycetemcomitans and P. gingivalis did not significantly increase after a single session of use of interdental brushes.
Authors: Büin Adams; J Massimo Nunes; Martin J Page; Timothy Roberts; Jonathan Carr; Theo A Nell; Douglas B Kell; Etheresia Pretorius Journal: Front Aging Neurosci Date: 2019-08-27 Impact factor: 5.750