Charlène Herbemont1, Sarah Chekroune2, Sarah Bonan2, Isabelle Cedrin-Durnerin3, Alexandre Vivot4, Charlotte Sonigo5, Jeremy Boujenah6, Michael Grynberg7, Christophe Sifer8. 1. AP-HP, Service de Cytogénétique et Biologie de la Reproduction-CECOS, Hôpital Jean Verdier, Bondy, France; Université, Paris XIII, Bobigny, France. 2. AP-HP, Service de Cytogénétique et Biologie de la Reproduction-CECOS, Hôpital Jean Verdier, Bondy, France. 3. AP-HP, Service de Médecine de la Reproduction et Préservation de la Fertilité, Hôpital Jean Verdier, Bondy, France. 4. AP-HP, Centre d'Epidémiologie Clinique, Hôpital Hôtel-Dieu, Paris, France; INSERM, UMR1153, Université Paris Descartes, Paris, France. 5. AP-HP, Service de Médecine de la Reproduction et Préservation de la Fertilité, Hôpital Jean Verdier, Bondy, France; INSERM, U1185, Université Paris-Sud, Le Kremlin-Bicetre, France. 6. AP-HP, Service de Médecine de la Reproduction et Préservation de la Fertilité, Hôpital Jean Verdier, Bondy, France; Service de Gynécologie-Obstétrique, Hôpital Jean Verdier, Bondy, France. 7. Université, Paris XIII, Bobigny, France; AP-HP, Service de Médecine de la Reproduction et Préservation de la Fertilité, Hôpital Jean Verdier, Bondy, France; INSERM, U1133, Université, Paris-Diderot, Paris, France. 8. AP-HP, Service de Cytogénétique et Biologie de la Reproduction-CECOS, Hôpital Jean Verdier, Bondy, France; Université, Paris XIII, Bobigny, France. Electronic address: christophe.sifer@aphp.fr.
Abstract
OBJECTIVE: To determine whether post-warming culture duration (1 hour vs. 18 hours) influences implantation rates (IRs) of good-quality blastocysts (GQB) in a good-prognosis population. DESIGN: Prospective interventional randomized study. SETTING:University hospital. PATIENT(S): One hundred sixty-two GQB transfers. INTERVENTION(S): Patients' vitrified blastocysts were randomly allocated to group A, warming on the day before transfer (n = 81), or B, warming on the day of transfer (n = 81). MAIN OUTCOME MEASURE(S): IR, live birth rate, reexpansion degree, and quality after warming and immediately before transfer. RESULT(S): Quality of the warmed and transferred blastocysts was similar (respectively, 39.1% and 32.7% top quality [≥B4AA/AB/BA] in group A vs. 41.7 and 42.2% in group B). In group A, 14 of 102 blastocysts (12.2%) appeared to be unsuitable for transfer, versus only 1 of 103 (0.9%) in group B, thus leading to an additional warming. As expected, reexpansion degree just before transfer was higher in group A (0.90 vs. 0.70). Likewise, the proportion of hatched blastocysts before transfer was higher after a longer culture period (38.6% in group A vs. 12.7% in group B). IRs were similar (38.0% in group A vs. 36% in group B), as were live birth rates (35.8% in group A vs. 34.6% in group B). CONCLUSION(S): IRs were not different, whatever the duration of post-warming culture of GQB. Both warming strategies could be applied to good-prognosis patients to optimize the laboratory workflow without any detrimental effect.
RCT Entities:
OBJECTIVE: To determine whether post-warming culture duration (1 hour vs. 18 hours) influences implantation rates (IRs) of good-quality blastocysts (GQB) in a good-prognosis population. DESIGN: Prospective interventional randomized study. SETTING: University hospital. PATIENT(S): One hundred sixty-two GQB transfers. INTERVENTION(S): Patients' vitrified blastocysts were randomly allocated to group A, warming on the day before transfer (n = 81), or B, warming on the day of transfer (n = 81). MAIN OUTCOME MEASURE(S): IR, live birth rate, reexpansion degree, and quality after warming and immediately before transfer. RESULT(S): Quality of the warmed and transferred blastocysts was similar (respectively, 39.1% and 32.7% top quality [≥B4AA/AB/BA] in group A vs. 41.7 and 42.2% in group B). In group A, 14 of 102 blastocysts (12.2%) appeared to be unsuitable for transfer, versus only 1 of 103 (0.9%) in group B, thus leading to an additional warming. As expected, reexpansion degree just before transfer was higher in group A (0.90 vs. 0.70). Likewise, the proportion of hatched blastocysts before transfer was higher after a longer culture period (38.6% in group A vs. 12.7% in group B). IRs were similar (38.0% in group A vs. 36% in group B), as were live birth rates (35.8% in group A vs. 34.6% in group B). CONCLUSION(S): IRs were not different, whatever the duration of post-warming culture of GQB. Both warming strategies could be applied to good-prognosis patients to optimize the laboratory workflow without any detrimental effect.
Authors: Ji Young Hwang; Jae Kyun Park; Tae Hyung Kim; Jin Hee Eum; HaengSeok Song; Jin Young Kim; Han Moie Park; Chan Woo Park; Woo Sik Lee; Sang Woo Lyu Journal: Clin Exp Reprod Med Date: 2020-11-10
Authors: Luis H Sordia-Hernandez; Felipe A Morales Martinez; Eduardo Gutierrez Orozco; Andrea Flores-Rodriguez; Paloma C Leyva-Camacho; Neri Alejandro Alvarez-Villalobos; Jorge Alberto Zuñiga-Hernandez Journal: J Reprod Infertil Date: 2021 Apr-Jun