A coumarin-based fluorescent probe for ratiometric detection of hydrazine and its application in living cells.

A new ratiometric fluorescent probe (1) was developed for the detection of hydrazine. The probe was obtained by incorporating the recognition moiety of acetyl group onto a coumarin fluorophore. Probe 1 displayed a distinct cyan emission in a 100% aqueous phosphate buffer solution. In the presence of hydrazine, probe 1 undergoes a hydrazinolysis process to release the coumarin fluorophore, which exhibited significant hypsochromic shifts in both absorption and emission spectra, and thus achieving a ratiometric response. This ratiometric probe is highly selective and sensitive towards hydrazine detection. The limit of detection (LOD) was calculated to be 34 nM. Moreover, cellular toxicity and imaging experiments suggested that probe 1 is can be used to monitor hydrazine in live cells.