Thaís Fonte-Faria1, Marta Citelli2, Georgia C Atella3, Helena Fonseca Raposo4, Lilia Zago5, Thamiris de Souza5, Simone Vargas da Silva1, Christina Barja-Fidalgo1. 1. Departamento de Biologia Celular, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil. 2. Instituto de Nutrição, Departamento de Nutrição Básica e Experimental, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: martacitelli@gmail.com. 3. Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. 4. Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas, São Paulo, Brazil. 5. Instituto de Nutrição, Departamento de Nutrição Básica e Experimental, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Abstract
OBJECTIVE: Chia seed oil is the richest source of plant-based ω-3 fatty acid, α-linolenic acid, but its potential and mechanisms of action to treat obesity are unclear. The aim of the study was to evaluate the effects of chia oil (ChOi) supplementation on body composition and insulin signaling in skeletal muscles of obese mice. METHODS: Male C57 BL/6 mice (n = 8/group) were fed regular control chow or a high-fat diet (HFD) for 135 d. Another HFD group additionally received ChOi from 90 to 135 d. RESULTS: Consumption of ChOi reduced fat mass accumulation and increased lean mass as evidenced by nuclear magnetic resonance. Moreover, obese mice treated with ChOi showed higher tyrosine phosphorylation of insulin receptor substrate 1, greater activation of protein kinase B, and increased translocation of glucose transporter type 4 in skeletal muscle tissue in response to insulin. ChOi supplementation improved glucose levels and insulin tolerance; decreased serum insulin, leptin, and triacylglycerols; and increased blood high-density lipoprotein cholesterol levels. All these effects caused by the use of ChOi seemed to be independent of the resolution of inflammation because the markers of inflammation were not altered in animals fed the HFD. CONCLUSION: The molecular effects observed in muscle tissue together with changes in body composition may have contributed to the increased glucose tolerance and to the healthy phenotype presented by obese animals treated with ChOi.
OBJECTIVE:Chia seed oil is the richest source of plant-based ω-3 fatty acid, α-linolenic acid, but its potential and mechanisms of action to treat obesity are unclear. The aim of the study was to evaluate the effects of chia oil (ChOi) supplementation on body composition and insulin signaling in skeletal muscles of obesemice. METHODS: Male C57 BL/6 mice (n = 8/group) were fed regular control chow or a high-fat diet (HFD) for 135 d. Another HFD group additionally received ChOi from 90 to 135 d. RESULTS: Consumption of ChOi reduced fat mass accumulation and increased lean mass as evidenced by nuclear magnetic resonance. Moreover, obesemice treated with ChOi showed higher tyrosine phosphorylation of insulin receptor substrate 1, greater activation of protein kinase B, and increased translocation of glucose transporter type 4 in skeletal muscle tissue in response to insulin. ChOi supplementation improved glucose levels and insulin tolerance; decreased serum insulin, leptin, and triacylglycerols; and increased blood high-density lipoprotein cholesterol levels. All these effects caused by the use of ChOi seemed to be independent of the resolution of inflammation because the markers of inflammation were not altered in animals fed the HFD. CONCLUSION: The molecular effects observed in muscle tissue together with changes in body composition may have contributed to the increased glucose tolerance and to the healthy phenotype presented by obese animals treated with ChOi.
Authors: Sara Motyka; Katarzyna Koc; Halina Ekiert; Eliza Blicharska; Katarzyna Czarnek; Agnieszka Szopa Journal: Molecules Date: 2022-02-11 Impact factor: 4.411
Authors: Luiza de Paula Dias Moreira; Bárbara Nery Enes; Vinícius Parzanini Brilhante de São José; Renata Celi Lopes Toledo; Luiz Carlos Maia Ladeira; Rodrigo Rezende Cardoso; Vinícius da Silva Duarte; Helen Hermana Miranda Hermsdorff; Frederico Augusto Ribeiro de Barros; Hércia Stampini Duarte Martino Journal: Foods Date: 2022-01-21