V Fuochi1, V Cardile2, G Petronio Petronio1, P M Furneri1. 1. Dipartimento di Scienze Biomediche e Biotecnologiche BIOMETEC Sez. Microbiologia, Università degli Studi di Catania, Catania, Italy. 2. Dipartimento di Scienze Biomediche e Biotecnologiche BIOMETEC Sez. Fisiologia, Università degli Studi di Catania, Catania, Italy.
Abstract
AIMS: The aim of this study was to characterize Lactobacillus strains for their biological properties and amensalistic activities against genital and nongenital pathogens. METHODS AND RESULTS: For the purpose, some special characteristics (H2 O2 , biofilm and antimicrobial substances production) as well as safety properties of 112 lactobacilli were evaluated. All the strains had good amensalistic characteristics, in particular cell-free supernatants of 10 strains showed antibacterial activity against bacteria, as well as Candida sp. Moreover, these 10 strains were excellent biofilm producers. CONCLUSIONS: These results provide evidence for the possible use as probiotics for vaginal co-therapy in case of dysbiosis. SIGNIFICANCE AND IMPACT OF THE STUDY: Recently, the problem of antibiotic resistance is constantly increasing, even though resources and energy are invested in order to increase knowledge on the mechanisms of action. Bacteriocins have a rapid mechanism of action, act at extremely low concentrations, are generally sensitive to proteases and they usually have a narrow killing spectrum; these characteristics reduce the possibility of the bacterium to develop resistance. This study is focused on the feasibility of a high production of antimicrobial substances and their characterization in order to be exploited as a therapeutic alterative or in co-therapy with antibiotics in case of vaginal dysbiosis.
AIMS: The aim of this study was to characterize Lactobacillus strains for their biological properties and amensalistic activities against genital and nongenital pathogens. METHODS AND RESULTS: For the purpose, some special characteristics (H2 O2 , biofilm and antimicrobial substances production) as well as safety properties of 112 lactobacilli were evaluated. All the strains had good amensalistic characteristics, in particular cell-free supernatants of 10 strains showed antibacterial activity against bacteria, as well as Candida sp. Moreover, these 10 strains were excellent biofilm producers. CONCLUSIONS: These results provide evidence for the possible use as probiotics for vaginal co-therapy in case of dysbiosis. SIGNIFICANCE AND IMPACT OF THE STUDY: Recently, the problem of antibiotic resistance is constantly increasing, even though resources and energy are invested in order to increase knowledge on the mechanisms of action. Bacteriocins have a rapid mechanism of action, act at extremely low concentrations, are generally sensitive to proteases and they usually have a narrow killing spectrum; these characteristics reduce the possibility of the bacterium to develop resistance. This study is focused on the feasibility of a high production of antimicrobial substances and their characterization in order to be exploited as a therapeutic alterative or in co-therapy with antibiotics in case of vaginal dysbiosis.
Authors: Miguel Carda-Diéguez; Nívia Cárdenas; Marina Aparicio; David Beltrán; Juan M Rodríguez; Alex Mira Journal: Front Med (Lausanne) Date: 2019-08-07