Utility of acidic xylanase of Bacillus subtilis subsp. subtilis JJBS250 in improving the nutritional value of poultry feed.

Cane molasses has been employed as a cost-effective medium for enhanced xylanase production in submerged fermentation. Bacillus subtilis subsp. subtilis JJBS250 produced xylanase (15.16 U/ml) at pH 4.0, 35 °C and 200 rpm after 54 h using optimized basal medium by 'one variable at a time approach'. Addition of Tween 80 and PEG 4000 also enhanced xylanase production in cane molasses medium. Combined effect of yeast extract, incubation time and PEG 4000 using statistical optimization enhanced xylanase production to 38.60 U/ml, which was 2.54-fold higher than the 'one variable at a time approach'. The efficacy of xylanase from Bacillus subtilis subsp. subtilis JJBS 250 was evaluated in the improvement of poultry feed nutrition. Xylanase addition (10 IU/g feed) enhanced liberation of reducing sugars (95.540 mg/g substrate) after 48 h at 60 °C. Optimization has resulted in enhanced production of xylanase that improved the nutritional quality of poultry feed.