Literature DB >> 3049822

Assessment of phagocytosis of plastic adherent group B streptococci by ELISA quenching.

P Rainard1.   

Abstract

An ELISA was devised to quantitate the number of group B streptococci (GBS) adherent to flat-bottomed 96-well microtiter plates. Ingestion of GBS by bovine polymorphonuclears (PMN) led to a decrease in ELISA values. This ELISA quenching proved to be proportional to phagocytosis. The method is suitable for kinetic studies of phagocytosis using glutaraldehyde to block the uptake of bacteria. The ELISA quenching test is applicable to a large number of samples and permits the use of the semi-automated equipment developed for ELISA.

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Year:  1988        PMID： 3049822     DOI： 10.1016/0022-1759(88)90389-4

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  2 in total

1.  The kinetics of inflammation and phagocytosis during bovine mastitis induced by Streptococcus agalactiae bearing the protein X.

Authors:  P Rainard; Y Lautrou; P Sarradin; A Coulibaly; B Poutrel
Journal:  Vet Res Commun       Date:  1991       Impact factor: 2.459

2.  Protein X of Streptococcus agalactiae induces opsonic antibodies in cows.

Authors:  P Rainard; Y Lautrou; P Sarradin; B Poutrel
Journal:  J Clin Microbiol       Date:  1991-09       Impact factor: 5.948
  2 in total

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