Literature DB >> 3049254

The primary structure of the operons coding for Shigella dysenteriae toxin and temperate phage H30 shiga-like toxin.

Yu V Kozlov1, A A Kabishev, E V Lukyanov, A A Bayev.   

Abstract

Nucleotide(nt) sequences were determined for the toxin (SHT) operon present in the chromosome of Shigella dysenteriae 1 and for the shiga-like toxin (SLT) operon found in the lambdoid phage H30 genome. The coding sequences of the sht and slt genes differ in 4 nt with 1 nt change responsible for an amino acid replacement. The deduced amino acid sequence in the A chain of the toxins is highly homologous to that of the A chain of ricin, a plant toxin. SHT-coding mRNAs were detected by mapping the 5' termini and using blot-hybridisation; one of them was more abundant and coded only for the B subunit of SHT while the other (bi-cistronic mRNA) encoded both subunits. An IS element related to the IS3 element of Escherichia coli was found in the chromosome of S. dysenteriae near the sht operon.

Entities:  

Keywords:  Recombinant DNA; mapping of 5'-end mRNA; IS element; primer extension

Mesh：

Substances：

Year:  1988        PMID： 3049254     DOI： 10.1016/0378-1119(88)90398-8

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  15 in total

1.  Identification of a B subunit gene promoter in the Shiga toxin operon of Shigella dysenteriae 1.

Authors:  N F Habib; M P Jackson
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

2.  A system for production and rapid purification of large amounts of the Shiga toxin/Shiga-like toxin I B subunit.

Authors:  S B Calderwood; D W Acheson; M B Goldberg; S A Boyko; A Donohue-Rolfe
Journal:  Infect Immun       Date:  1990-09       Impact factor: 3.441

Review 3.  Genetic basis of virulence in Shigella species.

Authors:  T L Hale
Journal:  Microbiol Rev       Date:  1991-06

4.  Monoclonal antibody to Shiga toxin 2 which blocks receptor binding and neutralizes cytotoxicity.

Authors:  H Nakao; N Kiyokawa; J Fujimoto; S Yamasaki; T Takeda
Journal:  Infect Immun       Date:  1999-11       Impact factor: 3.441

5.  Transcription of the Shiga-like toxin type II and Shiga-like toxin type II variant operons of Escherichia coli.

Authors:  L M Sung; M P Jackson; A D O'Brien; R K Holmes
Journal:  J Bacteriol       Date:  1990-11       Impact factor: 3.490

6.  Use of a novel approach, termed island probing, identifies the Shigella flexneri she pathogenicity island which encodes a homolog of the immunoglobulin A protease-like family of proteins.

Authors:  K Rajakumar; C Sasakawa; B Adler
Journal:  Infect Immun       Date:  1997-11       Impact factor: 3.441

7.  Identification, characterization, and distribution of a Shiga toxin 1 gene variant (stx(1c)) in Escherichia coli strains isolated from humans.

Authors:  Wenlan Zhang; Martina Bielaszewska; Thorsten Kuczius; Helge Karch
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

8.  Minimum domain of the Shiga toxin A subunit required for enzymatic activity.

Authors:  J E Haddad; A Y al-Jaufy; M P Jackson
Journal:  J Bacteriol       Date:  1993-08       Impact factor: 3.490

9.  Retrograde transport from the Golgi complex to the ER of both Shiga toxin and the nontoxic Shiga B-fragment is regulated by butyric acid and cAMP.

Authors:  K Sandvig; M Ryd; O Garred; E Schweda; P K Holm; B van Deurs
Journal:  J Cell Biol       Date:  1994-07       Impact factor: 10.539

10.  Roles of a ribosome-binding site and mRNA secondary structure in differential expression of Shiga toxin genes.

Authors:  N F Habib; M P Jackson
Journal:  J Bacteriol       Date:  1993-02       Impact factor: 3.490
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