Bruno Ramos-Molina1,2, Daniel Castellano-Castillo1,2, Oscar Pastor3, Luis Ocaña-Wilhelmi4, Diego Fernández-García5, Manuel Romero-Gómez6, Fernando Cardona7,8, Francisco J Tinahones1,2. 1. Unidad Gestión Clínica Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Campus de Teatinos s/n, 29010, Malaga, Spain. 2. CIBER Fisiopatología de la Obesidad y Nutrición (CIBERObn), Instituto de Salud Carlos III, Madrid, Spain. 3. Unidad de Cuantificación y Caracterización Molecular, Servicio de Bioquímica Clínica, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Universitario Ramón y Cajal, Madrid, Spain. 4. Unidad de Cirugía Metabólica, Hospital Universitario Virgen de la Victoria, Málaga, Spain. 5. Unidad Gestión Clínica Endocrinología y Nutrición, Hospital Universitario Virgen de la Victoria, Málaga, Spain. 6. Unidad de Gestión Clínica de Aparato Digestivo, CIBERehd, Instituto de Biomedicina de Sevilla, Hospital Universitario Virgen del Rocío, Universidad de Sevilla, Sevilla, Spain. 7. Unidad Gestión Clínica Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Campus de Teatinos s/n, 29010, Malaga, Spain. fernandocardonadiaz@gmail.com. 8. CIBER Fisiopatología de la Obesidad y Nutrición (CIBERObn), Instituto de Salud Carlos III, Madrid, Spain. fernandocardonadiaz@gmail.com.
Abstract
BACKGROUND: Non-alcoholic steatohepatitis (NASH) is present in a high percentage of obese patients undergoing bariatric surgery. A significant proportion of patients still present NASH even after considerable weight loss and metabolic improvements after surgery. OBJECTIVE: To determine whether the changes in the serum lipidome after sleeve gastrectomy could be used to discriminate obese patients with NASH patients to those with non-alcoholic fatty liver (NAFL). METHODS: This study involved 24 patients with grade 3 obesity diagnosed with either NAFL (n = 8) or NASH (n = 16) using the non-invasive OWLiver assay. All patients suffering from NASH were re-evaluated 6 months after bariatric surgery using the OWLiver test to confirm NASH resolution. Serum lipid extracts were assessed at baseline and 6 months post surgery and were analyzed in an ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC-TOF-MS)-based platform. RESULTS: Lipidomic analysis revealed a differential sphingomyelin profile in patients with NASH resolution after sleeve gastrectomy. Certain serum sphingomyelin species were significantly higher at baseline in NASH patients in comparison to those with NAFL. Sphingomyelin profile of subjects with NASH resolution was similar to that for obese subjects with NAFL before bariatric surgery. CONCLUSION: Our study indicates that the serum sphingomyelin levels could be related to the status of non-alcoholic fatty liver disease and that certain sphingomyelin species may be used for the follow-up of obese patients with NASH after sleeve gastrectomy.
BACKGROUND:Non-alcoholic steatohepatitis (NASH) is present in a high percentage of obesepatients undergoing bariatric surgery. A significant proportion of patients still present NASH even after considerable weight loss and metabolic improvements after surgery. OBJECTIVE: To determine whether the changes in the serum lipidome after sleeve gastrectomy could be used to discriminate obesepatients with NASH patients to those with non-alcoholic fatty liver (NAFL). METHODS: This study involved 24 patients with grade 3 obesity diagnosed with either NAFL (n = 8) or NASH (n = 16) using the non-invasive OWLiver assay. All patients suffering from NASH were re-evaluated 6 months after bariatric surgery using the OWLiver test to confirm NASH resolution. Serum lipid extracts were assessed at baseline and 6 months post surgery and were analyzed in an ultra-performance liquid chromatography/time-of-flight mass spectrometry (UPLC-TOF-MS)-based platform. RESULTS: Lipidomic analysis revealed a differential sphingomyelin profile in patients with NASH resolution after sleeve gastrectomy. Certain serum sphingomyelin species were significantly higher at baseline in NASH patients in comparison to those with NAFL. Sphingomyelin profile of subjects with NASH resolution was similar to that for obese subjects with NAFL before bariatric surgery. CONCLUSION: Our study indicates that the serum sphingomyelin levels could be related to the status of non-alcoholic fatty liver disease and that certain sphingomyelin species may be used for the follow-up of obesepatients with NASH after sleeve gastrectomy.
Authors: Piotr Kalinowski; Rafał Paluszkiewicz; Bogna Ziarkiewicz-Wróblewska; Tadeusz Wróblewski; Piotr Remiszewski; Mariusz Grodzicki; Marek Krawczyk Journal: Ann Surg Date: 2017-11 Impact factor: 12.969
Authors: Ben Jones; Caroline Sands; Kleopatra Alexiadou; James Minnion; George Tharakan; Preeshila Behary; Ahmed R Ahmed; Sanjay Purkayastha; Matthew R Lewis; Stephen Bloom; Jia V Li; Tricia M Tan Journal: J Clin Endocrinol Metab Date: 2022-01-18 Impact factor: 5.958