Yongjie Lu1, Yau Kei Chan1,2, Long Hei Lau1, Youchuang Chao1, Kendrick Co Shih3, Shiu Ming Lai3, David Wong2,4, Ho Cheung Shum1,5. 1. Department of Mechanical Engineering, Faculty of Engineering, University of Hong Kong, Hong Kong, Hong Kong SAR, China. 2. Department of Eye and Vision Science, University of Liverpool, Liverpool, UK. 3. Department of Ophthalmology, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, Hong Kong SAR, China. 4. St Paul's Eye Unit, Royal Liverpool University Hospital, Liverpool, UK. 5. HKU-Shenzhen Institute of Research and Innovation (HKU-SIRI), University of Hong Kong, Hong Kong, Hong Kong SAR, China.
Abstract
PURPOSE: Silicone oil (SiO) with additives of high-molecular-weight (HMW) SiO molecules, eases both the injection and removal. When used inside an eye, it remains unclear how increasing extensional viscosity of SiO might reduce emulsification. Using cell-lined models, this study aims to understand the reason why SiO with HMW is less prone to emulsification. METHODS: The adhesion of SiO was studied and recorded in a cell-coated microchannel by optical microscopy. The resistance of SiO against emulsification was tested on another cell-coated eye-on-a-chip platform, which was subject to simulated saccadic eye movements, for 4 days. Silicone oil (SiO) candidates with HMW, SiOHMW 2000 and SiOHMW 5000 , and their counterparts SiO2000 and SiO5000 without HMW, were tested. The quantity of the SiO emulsified droplets formed was assessed daily by optical microscopy. RESULTS: When flowing in the microchannel, SiO adheres on the cell-coated substrate. The number of droplets is generally lower in SiO with HMW than their counterparts. At the end of the experiment, the average numbers of droplets in SiO2000 (29.1 ± 41.0) and SiO5000 (9.1 ± 19.5) are higher than those in SiOHMW 2000 (6.0 ± 4.5) and SiOHMW 5000 (5.6 ± 4.1). CONCLUSION: A new mechanism of emulsification of SiO is proposed: SiO adheres to ocular tissue to form emulsified droplets. The presence of HMW, which increases the extensional viscosity, may resist the break-up of SiO from the substrate to form emulsified droplets. When tested in a physiologically representative platform, the use of HMW in SiO generally reduces the number of droplets formed in vitro.
PURPOSE:Silicone oil (SiO) with additives of high-molecular-weight (HMW) SiO molecules, eases both the injection and removal. When used inside an eye, it remains unclear how increasing extensional viscosity of SiO might reduce emulsification. Using cell-lined models, this study aims to understand the reason why SiO with HMW is less prone to emulsification. METHODS: The adhesion of SiO was studied and recorded in a cell-coated microchannel by optical microscopy. The resistance of SiO against emulsification was tested on another cell-coated eye-on-a-chip platform, which was subject to simulated saccadic eye movements, for 4 days. Silicone oil (SiO) candidates with HMW, SiOHMW 2000 and SiOHMW 5000 , and their counterparts SiO2000 and SiO5000 without HMW, were tested. The quantity of the SiO emulsified droplets formed was assessed daily by optical microscopy. RESULTS: When flowing in the microchannel, SiO adheres on the cell-coated substrate. The number of droplets is generally lower in SiO with HMW than their counterparts. At the end of the experiment, the average numbers of droplets in SiO2000 (29.1 ± 41.0) and SiO5000 (9.1 ± 19.5) are higher than those in SiOHMW 2000 (6.0 ± 4.5) and SiOHMW 5000 (5.6 ± 4.1). CONCLUSION: A new mechanism of emulsification of SiO is proposed: SiO adheres to ocular tissue to form emulsified droplets. The presence of HMW, which increases the extensional viscosity, may resist the break-up of SiO from the substrate to form emulsified droplets. When tested in a physiologically representative platform, the use of HMW in SiO generally reduces the number of droplets formed in vitro.
Authors: Maximilian Hammer; Sonja Schickhardt; Donald J Munro; Alexander Scheuerle; Christian S Mayer; Gerd U Auffarth Journal: Transl Vis Sci Technol Date: 2022-02-01 Impact factor: 3.283