Literature DB >> 30483000

LC-ESI-MS based characterisation of isoflavones in soybean (Glycine max (L.) Merr.) from India.

M K Akitha Devi1, S Sravan Kumar1, P Giridhar1.   

Abstract

A total of twenty-one soybean varieties were screened for their morphological characteristics followed by isoflavone content analysis by HPLC. The total isoflavone (TI) content was found within a wide range of 140.9-1048.6 μg/g of soy in different varieties. The highest isoflavone content was found in MAUS-2 followed by DS_2613 and lowest in Karunae (140.9 μg/g of soy). Various isoflavone forms were identified by LC-ESI+ MS. Significant differences in the isoflavone content were observed for all the aglycones and their glucoside conjugates as well as total daidzein, total genistein, total glycitein, and TI. A positive correlation between TI content and growth stages was found during the progression of seed development. An increase of 5.4-fold and 5.3-fold of TI concentration was observed for JS 335 and MAUS-2 respectively, from early to green mature (R5-R8) stage of bean development.

Entities:  

Keywords:  Electrospray ion mass spectrophotometer; High performance liquid chromatography; Isoflavones; Malonylglucosides; Soybean cultivars

Year:  2018        PMID: 30483000      PMCID: PMC6233424          DOI: 10.1007/s13197-018-3443-0

Source DB:  PubMed          Journal:  J Food Sci Technol        ISSN: 0022-1155            Impact factor:   2.701


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