Zong-Yen Wu1,2, Shu-Peng Ho2, Jan-Fang Cheng1, Kwong-Chung Tung2, Yu-Kai Hong3, Shi-Yu Chen3, Alexander Menzer4, Ying-Ju Chen5, Yao-Ting Huang4, Po-Yu Liu6,7,8,9. 1. Department of Energy, Joint Genome Institute, CA 94598, USA. 2. Department of Veterinary Medicine, National Chung Hsing University, Taichung 40227, Taiwan. 3. Department of Computer Science & Information Engineering, National Chung Cheng University, Chia-Yi 62102, Taiwan. 4. Department of Hygiene & Infection Prevention, University Medical Center of the Johannes Gutenberg University Mainz, Mainz 55131, Germany. 5. Department of Food & Nutrition, Providence University, Taichung 43301, Taiwan. 6. Department of Physical Therapy, Shu-Zen Junior College of Medicine & Management, Kaohsiung City 82144, Taiwan. 7. Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan. 8. PhD Program in Translational Medicine, National Chung Hsing University, Taichung 40227, Taiwan. 9. Department of Internal Medicine, Division of Infectious Diseases, Taichung Veterans General Hospital, Taichung 40705, Taiwan.
Abstract
AIM: To describe the genomic characteristics of seawater-borne hemolytic Shewanella algae and its resistance genes. MATERIALS & METHODS: Whole genome sequence of S. algae SYT3 was determined using llumina MiSeq platform. Multiple-database-based analysis was performed to identify the genetic background of its hemolytic activity and the antibiotic resistance genes. RESULTS: S. algae SYT3 possesses a homolog of the hly operon involved in the synthesis of hemolysin. We also identified candidate genes associated with resistance to β-lactam antibiotics (bla OXA-55) and fluoroquinolone (qnrA3). CONCLUSION: The study provides an insight into the hemolytic activity of S. algae. Our findings also suggested S. algae as a potential reservoir of antimicrobial resistance determinants.
AIM: To describe the genomic characteristics of seawater-borne hemolytic Shewanella algae and its resistance genes. MATERIALS & METHODS: Whole genome sequence of S. algae SYT3 was determined using llumina MiSeq platform. Multiple-database-based analysis was performed to identify the genetic background of its hemolytic activity and the antibiotic resistance genes. RESULTS:S. algae SYT3 possesses a homolog of the hly operon involved in the synthesis of hemolysin. We also identified candidate genes associated with resistance to β-lactam antibiotics (bla OXA-55) and fluoroquinolone (qnrA3). CONCLUSION: The study provides an insight into the hemolytic activity of S. algae. Our findings also suggested S. algae as a potential reservoir of antimicrobial resistance determinants.