Literature DB >> 3047397

A high resolution diffracting crystal form of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase.

M Ruff1, J Cavarelli, V Mikol, B Lorber, A Mitschler, R Giege, J C Thierry, D Moras.   

Abstract

Three new crystal forms of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase have been produced. The best crystals, obtained after modifying both purification and crystallization conditions, belong to space group P2(1)2(1)2(1) and diffract to 2.7 A. Unit cell parameters are a = 210.4 A, b = 145.3 A and c = 86.0 A (1 A = 0.1 nm), with one dimeric enzyme and two tRNA molecules in the asymmetric unit.

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Year:  1988        PMID： 3047397     DOI： 10.1016/0022-2836(88)90450-0

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  6 in total

1.  Crystal structure of aspartyl-tRNA synthetase from Pyrococcus kodakaraensis KOD: archaeon specificity and catalytic mechanism of adenylate formation.

Authors:  E Schmitt; L Moulinier; S Fujiwara; T Imanaka; J C Thierry; D Moras
Journal:  EMBO J       Date:  1998-09-01       Impact factor: 11.598

2.  New photoactivatable structural and affinity probes of RNAs: specific features and applications for mapping of spermine binding sites in yeast tRNA(Asp) and interaction of this tRNA with yeast aspartyl-tRNA synthetase.

Authors:  A Garcia; R Giegé; J P Behr
Journal:  Nucleic Acids Res       Date:  1990-01-11       Impact factor: 16.971

3.  A domain in the N-terminal extension of class IIb eukaryotic aminoacyl-tRNA synthetases is important for tRNA binding.

Authors:  M Frugier; L Moulinier; R Giegé
Journal:  EMBO J       Date:  2000-05-15       Impact factor: 11.598

4.  Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterisation of the gene, homologies of its translated amino acid sequence with asparaginyl- and lysyl-tRNA synthetases.

Authors:  G Eriani; G Dirheimer; J Gangloff
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

5.  Role of dimerization in yeast aspartyl-tRNA synthetase and importance of the class II invariant proline.

Authors:  G Eriani; J Cavarelli; F Martin; G Dirheimer; D Moras; J Gangloff
Journal:  Proc Natl Acad Sci U S A       Date:  1993-11-15       Impact factor: 11.205

6.  The active site of yeast aspartyl-tRNA synthetase: structural and functional aspects of the aminoacylation reaction.

Authors:  J Cavarelli; G Eriani; B Rees; M Ruff; M Boeglin; A Mitschler; F Martin; J Gangloff; J C Thierry; D Moras
Journal:  EMBO J       Date:  1994-01-15       Impact factor: 11.598
  6 in total

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