Jake A Lacey1, Dragana Stanley2, Anthony L Keyburn3, Mark Ford4, Honglei Chen4, Priscilla Johanesen5, Dena Lyras5, Robert J Moore6. 1. Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, VIC, 3800, Australia; CSIRO Biosecurity Flagship, Australian Animal Health Laboratory, Geelong, VIC, 3800, Australia; Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia. 2. CSIRO Biosecurity Flagship, Australian Animal Health Laboratory, Geelong, VIC, 3800, Australia; Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia; Central Queensland University, School of Medical and Applied Sciences, Rockhampton, QLD, 4702, Australia. 3. CSIRO Biosecurity Flagship, Australian Animal Health Laboratory, Geelong, VIC, 3800, Australia; Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia. 4. CSIRO Biosecurity Flagship, Australian Animal Health Laboratory, Geelong, VIC, 3800, Australia. 5. Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, VIC, 3800, Australia. 6. Infection and Immunity Program, Monash Biomedicine Discovery Institute and Department of Microbiology, Monash University, Clayton, VIC, 3800, Australia; CSIRO Biosecurity Flagship, Australian Animal Health Laboratory, Geelong, VIC, 3800, Australia; Poultry Cooperative Research Centre, University of New England, Armidale, NSW, 2351, Australia; School of Science, RMIT University, Bundoora, VIC, 3800, Australia. Electronic address: rob.moore@rmit.edu.au.
Abstract
PROBLEM ADDRESSED: Clostridium perfringens is the etiological agent of necrotic enteritis in chickens. As necrotic enteritis is a gastrointestinal disease, the interactions of pathogenic C. perfringens strains with the complex microbiota of the gastrointestinal tract may influence disease development and severity of disease. OBJECTIVE: In this study the interactions of a pathogenic strain of C. perfringens, WER-NE36, with the microbiota of broilers was investigated to determine whether the pre-existing microbiota could influence disease outcomes in the necrotic enteritis challenge model. Methods and approach: Faecal microbiota compositions were measured before and after C. perfringens challenge and caecal microbiota was also characterised at necropsy. The microbiota profiles from individual birds were related back to the degree of necrotic enteritis that each bird developed. RESULTS: Under the experimental conditions used the pre-existing microbiota did not have an effect on disease outcomes. However, C. perfringens challenge was shown to have a significant effect on the microbiota of broilers, regardless of disease status, by displacement of commensal clostridia. CONCLUSIONS: The microbiota signature after challenge resembled that of lower productivity birds, supporting the finding that physically obvious disease (necrotic lesions), as well as dysbiosis, are associated with shifts in gut microbiota and affect broiler performance, increasing costs to the poultry industry.
PROBLEM ADDRESSED: Clostridium perfringens is the etiological agent of necrotic enteritis in chickens. As necrotic enteritis is a gastrointestinal disease, the interactions of pathogenic C. perfringens strains with the complex microbiota of the gastrointestinal tract may influence disease development and severity of disease. OBJECTIVE: In this study the interactions of a pathogenic strain of C. perfringens, WER-NE36, with the microbiota of broilers was investigated to determine whether the pre-existing microbiota could influence disease outcomes in the necrotic enteritis challenge model. Methods and approach: Faecal microbiota compositions were measured before and after C. perfringens challenge and caecal microbiota was also characterised at necropsy. The microbiota profiles from individual birds were related back to the degree of necrotic enteritis that each bird developed. RESULTS: Under the experimental conditions used the pre-existing microbiota did not have an effect on disease outcomes. However, C. perfringens challenge was shown to have a significant effect on the microbiota of broilers, regardless of disease status, by displacement of commensal clostridia. CONCLUSIONS: The microbiota signature after challenge resembled that of lower productivity birds, supporting the finding that physically obvious disease (necrotic lesions), as well as dysbiosis, are associated with shifts in gut microbiota and affect broiler performance, increasing costs to the poultry industry.
Authors: David Minich; Christopher Madden; Mauricio A Navarro; Leo Glowacki; Kristen French-Kim; Willow Chan; Morgan V Evans; Kilmer Soares; Ryan Mrofchak; Rushil Madan; Gregory A Ballash; Krista LaPerle; Subhadeep Paul; Yael Vodovotz; Francisco A Uzal; Margaret Martinez; Jennifer Hausmann; Randall E Junge; Vanessa L Hale Journal: Anim Microbiome Date: 2022-01-09
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