Nan Jiang1, Xinzhuo Zhang2, Yingshun Zhou3, Zhikun Zhang3, Xiaoli Zheng4. 1. School of International Education, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China; Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China; Department of Physiology, School of Basic Medicine, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China. 2. School of International Education, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China; Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China. Electronic address: zhangxinzhuo@swmu.edu.cn. 3. Department of Pathogenic Biology, School of Basic Medicine, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China. 4. Department of Physiology, School of Basic Medicine, Southwest Medical University, No. 1 Xianglin Road, Luzhou, Sichuan, China.
Abstract
OBJECTIVES: Acinetobacter spp. isolates carrying the blaNDM-1 gene are frequently reported. However, most reported blaNDM-1 genes are carried by clinical strains. Here we report a carbapenem-resistant Acinetobacter towneri isolate from hospital sewage in China co-harbouring blaNDM-1 and blaOXA-58 in the genome. METHODS: Whole-genome sequencing was performed using a single molecule, real-time (SMRT) sequencing platform with a Pacific Biosciences RS II Sequencer and MiSeq system. Reads were de novo assembled using Celera Assembler v.8.0. Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and the genome sequence was analysed by bioinformatics methods. RESULTS: The 2963729-bp genome with a G+C content of 41.30% displayed 11 antimicrobial resistance genes, including blaNDM-1 and blaOXA-58. Meanwhile, 2 plasmids and 19 genomic islands were predicted within the genome. CONCLUSION: The whole-genome sequence reported here can be compared with other genomes of NDM-1-producing Acinetobacter spp. These data could facilitate further understanding of the specific genomic features of carbapenem-resistant Acinetobacter spp. in China.
OBJECTIVES:Acinetobacter spp. isolates carrying the blaNDM-1 gene are frequently reported. However, most reported blaNDM-1 genes are carried by clinical strains. Here we report a carbapenem-resistant Acinetobacter towneri isolate from hospital sewage in China co-harbouring blaNDM-1 and blaOXA-58 in the genome. METHODS: Whole-genome sequencing was performed using a single molecule, real-time (SMRT) sequencing platform with a Pacific Biosciences RS II Sequencer and MiSeq system. Reads were de novo assembled using Celera Assembler v.8.0. Genome annotation was performed using the NCBI Prokaryotic Genome Annotation Pipeline (PGAP), and the genome sequence was analysed by bioinformatics methods. RESULTS: The 2963729-bp genome with a G+C content of 41.30% displayed 11 antimicrobial resistance genes, including blaNDM-1 and blaOXA-58. Meanwhile, 2 plasmids and 19 genomic islands were predicted within the genome. CONCLUSION: The whole-genome sequence reported here can be compared with other genomes of NDM-1-producing Acinetobacter spp. These data could facilitate further understanding of the specific genomic features of carbapenem-resistant Acinetobacter spp. in China.