Literature DB >> 3047160

A preliminary evaluation of five HIV antigen detection assays.

D S Healey1, W J Maskill, E V Neate, F Beaton, I D Gust.   

Abstract

Five commercial assays for detecting HIV antigen were evaluated using a panel of 40 coded samples in six laboratories. All assays were capable of detecting HIV-1 antigen (HIV-1 Ag), and are likely to prove useful for monitoring supernatant fluids from a variety of cell cultures. The concentration of HIV-1 protein which the assays detected varied from 25 ng/ml down to 25 pg/ml. Three of the five assays were also able to detect HIV-2 Ag. More extensive evaluations are needed to determine the sensitivity and specificity of these assays on serum samples and body fluids.

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Year:  1988        PMID: 3047160     DOI: 10.1016/0166-0934(88)90145-0

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  8 in total

1.  Characterization of a discontinuous epitope of the human immunodeficiency virus (HIV) core protein p24 by epitope excision and differential chemical modification followed by mass spectrometric peptide mapping analysis.

Authors:  E O Hochleitner; C Borchers; C Parker; R J Bienstock; K B Tomer
Journal:  Protein Sci       Date:  2000-03       Impact factor: 6.725

2.  Evaluation of two enzyme immunoassays for detection of human immunodeficiency virus antigen in African and European sera.

Authors:  G Vercauteren; P Piot; M Vandenbruaene; G van der Groen
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-10       Impact factor: 3.267

3.  Comparison of core antigen (p24) assay and reverse transcriptase activity for detection of human immunodeficiency virus type 1 replication.

Authors:  S Land; F Beaton; D A McPhee; I D Gust
Journal:  J Clin Microbiol       Date:  1989-03       Impact factor: 5.948

4.  First documented case of human immunodeficiency virus type 2 infection in an asymptomatic Swiss subject.

Authors:  P Bürgisser; L Odier; J Schüpbach; P C Frei
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1989-10       Impact factor: 3.267

5.  Analysis of ovine lentivirus infectivity and replication by using a focal immunoassay and an antigen-capture enzyme-linked immunosorbent assay.

Authors:  K A Marcom; S J Brodie; L D Pearson; J C DeMartini
Journal:  J Clin Microbiol       Date:  1992-11       Impact factor: 5.948

6.  Negative regulation of human immune deficiency virus replication in monocytes. Distinctions between restricted and latent expression in THP-1 cells.

Authors:  J A Mikovits; M Gonda; M Ruta; N C Lohrey; H F Kung; F W Ruscetti
Journal:  J Exp Med       Date:  1990-05-01       Impact factor: 14.307

7.  Molecular specificity of two commercial enzyme linked immunosorbent assays for human immunodeficiency virus antigens.

Authors:  K E Brown; D C Shanson; P Mascagni; A R Coates
Journal:  J Clin Pathol       Date:  1991-12       Impact factor: 3.411

8.  Blind comparison of Abbott and Dupont HIV antigen ELISA tests for detecting antigenaemia in asymptomatic human immunodeficiency virus antibody positive homosexual men.

Authors:  D C Shanson; K E Brown; S Goonesekera; B Gazzard; A R Coates
Journal:  J Clin Pathol       Date:  1990-05       Impact factor: 3.411

  8 in total

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