Juanjuan Xu1, Yunguang He2, Jian Wang1, Xi Li1, Li Huang1, Shan Li3, Xue Qin4. 1. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. 2. Department of Endocrinology, Minzu Hospital of Guangxi Zhuang Autonomous Region, Affiliated Minzu Hospital of Guangxi Medical University, Nanning, Guangxi, China. 3. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. Electronic address: lis8858@126.com. 4. Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China. Electronic address: qinxue919@126.com.
Abstract
OBJECTIVES: The tumour necrosis factor superfamily 4 (TNFSF4) is a candidate gene for autoimmune diseases. We investigated the relationship of this gene with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) in a Chinese Guangxi population. METHODS: A total of 294 patients with SLE, 210 with RA, and 282 healthy controls were genotyped for single nucleotide polymorphism (SNP) rs1234315 using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The potential functional effects of the SNP were predicted by in silico analysis. RESULTS: Statistically significant associations with SLE and RA were detected at rs1234315, both by allele analysis (odds ratio 1.47, 95% confidence interval 1.17-1.86, p = 0.001; odds ratio 1.49, 95% confidence interval 1.15-1.92, p = 0.002; respectively), and genotype analysis (p = 0.003 and p = 7.000 × 10-5, respectively). The Akaike's information criterion (AIC) values indicated that the recessive model may serve as the best-fit model for the SNP for SLE and RA. CONCLUSION: Our results provided support for TNFSF4 rsl234315 as a SLE and RA susceptibility locus in a Chinese Guangxi population.
OBJECTIVES: The tumour necrosis factor superfamily 4 (TNFSF4) is a candidate gene for autoimmune diseases. We investigated the relationship of this gene with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) in a Chinese Guangxi population. METHODS: A total of 294 patients with SLE, 210 with RA, and 282 healthy controls were genotyped for single nucleotide polymorphism (SNP) rs1234315 using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The potential functional effects of the SNP were predicted by in silico analysis. RESULTS: Statistically significant associations with SLE and RA were detected at rs1234315, both by allele analysis (odds ratio 1.47, 95% confidence interval 1.17-1.86, p = 0.001; odds ratio 1.49, 95% confidence interval 1.15-1.92, p = 0.002; respectively), and genotype analysis (p = 0.003 and p = 7.000 × 10-5, respectively). The Akaike's information criterion (AIC) values indicated that the recessive model may serve as the best-fit model for the SNP for SLE and RA. CONCLUSION: Our results provided support for TNFSF4 rsl234315 as a SLE and RA susceptibility locus in a Chinese Guangxi population.
Authors: Mario Adán Moreno-Eutimio; Carmen Estefanía Martínez-Alemán; Ivan Sammir Aranda-Uribe; Guillermo Aquino-Jarquin; Carlos Cabello-Gutierrez; José Manuel Fragoso; Rosa Elda Barbosa-Cobos; Miguel A Saavedra; Julian Ramírez-Bello Journal: Clin Rheumatol Date: 2020-08-18 Impact factor: 2.980