Literature DB >> 30467549

Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans.

Alejandro Mata-Cabana1, Olga Sin2,3, Renée I Seinstra4, Ellen A A Nollen4.   

Abstract

C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.

Entities:  

Keywords:  C. elegans; Cytoplasm; Immunoblot; Nucleus; Protein localization; Subcellular fractionation

Year:  2018        PMID： 30467549      PMCID： PMC6245569          DOI： 10.21769/BioProtoc.3053

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

1.  Translocation of C. elegans CED-4 to nuclear membranes during programmed cell death.

Authors:  F Chen; B M Hersh; B Conradt; Z Zhou; D Riemer; Y Gruenbaum; H R Horvitz
Journal:  Science       Date:  2000-02-25       Impact factor: 47.728

2.  Alpha-synuclein acts in the nucleus to inhibit histone acetylation and promote neurotoxicity.

Authors:  Eirene Kontopoulos; Jeffrey D Parvin; Mel B Feany
Journal:  Hum Mol Genet       Date:  2006-09-07       Impact factor: 6.150

3.  Cytoplasmic mislocalization of TDP-43 is toxic to neurons and enhanced by a mutation associated with familial amyotrophic lateral sclerosis.

Authors:  Sami J Barmada; Gaia Skibinski; Erica Korb; Elizabeth J Rao; Jane Y Wu; Steven Finkbeiner
Journal:  J Neurosci       Date:  2010-01-13       Impact factor: 6.167

4.  Identification of an RNA Polymerase III Regulator Linked to Disease-Associated Protein Aggregation.

Authors:  Olga Sin; Tristan de Jong; Alejandro Mata-Cabana; Michelle Kudron; Mohamad Amr Zaini; Francesco A Aprile; Renée I Seinstra; Esther Stroo; Roméo Willinge Prins; Céline N Martineau; Hai Hui Wang; Wytse Hogewerf; Anne Steinhof; Erich E Wanker; Michele Vendruscolo; Cornelis F Calkhoven; Valerie Reinke; Victor Guryev; Ellen A A Nollen
Journal:  Mol Cell       Date:  2017-03-16       Impact factor: 17.970

5.  A protein nuclear extract from D. melanogaster larval tissues.

Authors:  Gaspare La Rocca; Giosalba Burgio; Davide F V Corona
Journal:  Fly (Austin)       Date:  2007-11-10       Impact factor: 2.160
  5 in total
  2 in total

1.  A novel in vitro Caenorhabditis elegans transcription system.

Authors:  Phillip Wibisono; Yiyong Liu; Jingru Sun
Journal:  BMC Mol Cell Biol       Date:  2020-11-30

2.  Location-specific inhibition of Akt reveals regulation of mTORC1 activity in the nucleus.

Authors:  Xin Zhou; Yanghao Zhong; Olivia Molinar-Inglis; Maya T Kunkel; Mingyuan Chen; Tengqian Sun; Jiao Zhang; John Y-J Shyy; JoAnn Trejo; Alexandra C Newton; Jin Zhang
Journal:  Nat Commun       Date:  2020-11-30       Impact factor: 14.919
  2 in total

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