A novel recombinant genome of minute virus of canines in China.

In this study, we determined almost all of the genome sequence of minute virus of canines (MVC) strain CDK47/2017 and performed phylogenetic analysis with this isolate and other MVC isolates. The genome of CDK47/2017 has the following characteristics: 1) The amino acid sequence of the NS1 protein is similar to that of the novel strain 15D009/KT241026.1, which has 17 identical amino acid changes and two identical amino acid insertions compared with other known MVC strains. These two strains clustered in a unique branch in an NSI-based phylogenetic tree. 2) Phylogenetic analysis based on the NP1 protein showed that strain CDK47/2017clustered in an independent branch with strains 15D009/KT241026.1 and HM-6/AB158475.1, both of which has 10 identical amino acid changes compared with other known MVC strains. 3) Eight unique amino acid substitutions of the CDK47/2017 capsid protein resulted in it forming a unique branch in the phylogenetic tree. 4) Recombination events were identified between the 3' end of the NS1 gene and 5' end of NP1 gene. Together, these characteristics suggest that strain CDK47/2017 represents a novel MVC strain that is distinct from all known MVC strains with sequences in the GenBank database. This contributes to a greater understanding of the genetic evolution of MVC.