Literature DB >> 30462348

Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics.

Linda Cerofolini1, Stefano Giuntini1,2, Azzurra Carlon1, Enrico Ravera1,2, Vito Calderone1,2, Marco Fragai1,2, Giacomo Parigi1,2, Claudio Luchinat1,2.   

Abstract

Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.
© 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Entities:  

Keywords:  PEGylation; biopharmaceuticals; protein modifications; protein structures; structural biology

Mesh：

Substances：

Year:  2019        PMID： 30462348     DOI： 10.1002/chem.201804488

Source DB:  PubMed          Journal:  Chemistry        ISSN： 0947-6539            Impact factor:   5.236


  12 in total

1.  Atomic-Scale View of Protein-PEG Interactions that Redirect the Thermal Unfolding Pathway of PEGylated Human Galectin-3.

Authors:  Amanda Pritzlaff; Guillaume Ferré; Emma Mulry; Ling Lin; Niloofar Gopal Pour; Daniel A Savin; Michael E Harris; Matthew T Eddy
Journal:  Angew Chem Int Ed Engl       Date:  2022-08-25       Impact factor: 16.823

Review 2.  Integrative Approaches in Structural Biology: A More Complete Picture from the Combination of Individual Techniques.

Authors:  Linda Cerofolini; Marco Fragai; Enrico Ravera; Christoph A Diebolder; Ludovic Renault; Vito Calderone
Journal:  Biomolecules       Date:  2019-08-14

3.  Opportunistic complexes of E. coli L-asparaginases with citrate anions.

Authors:  Jacek Lubkowski; Waikin Chan; Alexander Wlodawer
Journal:  Sci Rep       Date:  2019-07-30       Impact factor: 4.379

Review 4.  From Synthesis to Characterization of Site-Selective PEGylated Proteins.

Authors:  Lisandra Herrera Belén; Carlota de Oliveira Rangel-Yagui; Jorge F Beltrán Lissabet; Brian Effer; Manuel Lee-Estevez; Adalberto Pessoa; Rodrigo L Castillo; Jorge G Farías
Journal:  Front Pharmacol       Date:  2019-12-18       Impact factor: 5.810

5.  Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase α-aminoacrylate intermediate.

Authors:  Jacob B Holmes; Viktoriia Liu; Bethany G Caulkins; Eduardo Hilario; Rittik K Ghosh; Victoria N Drago; Robert P Young; Jennifer A Romero; Adam D Gill; Paul M Bogie; Joana Paulino; Xiaoling Wang; Gwladys Riviere; Yuliana K Bosken; Jochem Struppe; Alia Hassan; Jevgeni Guidoulianov; Barbara Perrone; Frederic Mentink-Vigier; Chia-En A Chang; Joanna R Long; Richard J Hooley; Timothy C Mueser; Michael F Dunn; Leonard J Mueller
Journal:  Proc Natl Acad Sci U S A       Date:  2022-01-11       Impact factor: 11.205

6.  Revealing Escherichia coli type II L-asparaginase active site flexible loop in its open, ligand-free conformation.

Authors:  Maristella Maggi; Massimiliano Meli; Giorgio Colombo; Claudia Scotti
Journal:  Sci Rep       Date:  2021-09-23       Impact factor: 4.379

Review 7.  Structural and biophysical aspects of l-asparaginases: a growing family with amazing diversity.

Authors:  Joanna I Loch; Mariusz Jaskolski
Journal:  IUCrJ       Date:  2021-06-30       Impact factor: 4.769

8.  Improving the Treatment of Acute Lymphoblastic Leukemia.

Authors:  Ashish Radadiya; Wen Zhu; Adriana Coricello; Stefano Alcaro; Nigel G J Richards
Journal:  Biochemistry       Date:  2020-08-23       Impact factor: 3.162

9.  A protocol to automatically calculate homo-oligomeric protein structures through the integration of evolutionary constraints and NMR ambiguous contacts.

Authors:  Davide Sala; Linda Cerofolini; Marco Fragai; Andrea Giachetti; Claudio Luchinat; Antonio Rosato
Journal:  Comput Struct Biotechnol J       Date:  2019-12-26       Impact factor: 7.271

10.  Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for Burkholderia ambifaria lectin (BambL).

Authors:  Sakonwan Kuhaudomlarp; Linda Cerofolini; Sabrina Santarsia; Emilie Gillon; Silvia Fallarini; Grazia Lombardi; Maxime Denis; Stefano Giuntini; Carolina Valori; Marco Fragai; Anne Imberty; Alessandro Dondoni; Cristina Nativi
Journal:  Chem Sci       Date:  2020-10-21       Impact factor: 9.825
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